Heraeus Multifuge X3 Heraeus Multifuge X3R

Contains

• “Before Setting up” on page 2-2 • “Transporting the Centrifuge” on page 2-2 • “Location” on page 2-2 • “Aligning the Centrifuge” on page 2-3 • “Mains Connection” on page 2-3 • “Control Panel” on page 3-2 • “Keys” on page 3-2 Thermo Scientific 3 Multifuge X3 / X3 R 3-1 3 Control Panel Control Panel The control panel contains the keys and displays of the centrifuge (only the power switch is located on the back of the device). All parameters can be selected and changed during operation. Program selection keys Display numeric pad PULSE OPEN Directory key SPEED TIME TEMP ENTER START STOP Bucket Braking and Acceleration Key Changing the display mode Speed / RCF Value Changing the display mode chamber / sample temperature Changing the display mode Running time / Remaining Running time Keys The keys allow user intervention for controlling the operating mode as follows: Key Display contents Normal start of the centrifuge Start End run manually Stop Automatic release (possible only when device is switched on). Emergency Open lid release (see “Mechanical Emergency Door Release” on page 7-2) By pressing the key PULSE the centrifuge starts immediately and Pulse accelerates up to the end speed. Releasing the key initiates a stopping process at the highest braking curves. Use the directory key in order to modify the displayed value Directory Use the bucket key in order to have all available bucket types Bucket displayed in succession. Braking and Use the ACC DEC key to toggle between braking and acceleration Acceleration Curves curves. Use the Toggle key to change the display mode. (speed / RCF value, Changing the display sample / chamber temperature, run time counter from start or preset mode speed on) 3-2 Multifuge X3 / X3 R Thermo Scientific 4 Operation • “Switch on Centrifuge” on page 4-2 • “Lid Opening” on page 4-2 • “Rotor Installation” on page 4-2 • “Entering Parameters” on page 4-4 • “Centrifugation” on page 4-10 • “Temperature Adaptation during Standstill” on page 4-11 • “Short-term Centrifugation” on page 4-11 • “Removing the Rotor” on page 4-11 • “Aligning the Centrifuge” on page 4-12 Thermo Scientific Multifuge X3 / X3 R 4-1 4 Operation Switch on Centrifuge 1. Turn on the power switch on the back of the device. The device performs a self-check of its software. a. When the centrifuge lid is closed the following display shows: The top line shows the speed and the time. Both lines display 0. The temperature indicator displays the current temperature inside the rotor chamber. The bottom line shows the values for the last run. b. When the centrifuge lid is open the following display shows: The top line shows the message "Lid open". The bottom line shows the values for the last run. Lid Opening 1. Press the OPEN key . WARNING Do not reach into the crack between the lid and the housing. The lid is drawn shut automatically. Use the emergency release only for malfunctions and power failures (see “Mechanical Emergency Door Release” on page 7-2). Close Lid 2. Close the lid by pressing down on it lightly in the middle or on both sides of it. Two locks close the lid completely. Note The lid should audibly click into place. Rotor Installation The approved rotors for the Heraeus Multifuge X3 / X3R are listed in section “Rotor Selection” on page 1-6. Use only the rotors and accessories from this list in the centrifuge. 4-2 Multifuge X3 / X3 R Thermo Scientific 4 Operation Rotor Installation CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge. The centrifuge is equipped with an AutoLockTM locking system. This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted on to the centrifuge spindle. Proceed as follows: 1. Open the lid of the centrifuge and if necessary remove any dust, foreign objects or residue from the chamber. AutoLockTM and o-ring must be clean and undamaged. AutoLockTM O-Ring Figure 4-1. AutoLockTM 2. Place the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle. The rotor clicks automatically into place. CAUTION Do not force the rotor onto the centrifuge spindle. If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle with a bit of pressure. 3. Check if the rotor is properly installed by lifting it slightly on the handle. If the rotor can be pulled up, then it must be reclamped to the centrifuge spindle. WARNING If the rotor cannot be properly locked in place after several attempts, then the AutoLockTM is defective and you are not permitted to operate the rotor. Check for any damage to the rotor: Damaged rotors must not be used. Keep the centrifuge spindle area of the rotor clear of objects. CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle. Thermo Scientific Multifuge X3 / X3 R 4-3 4 Operation Entering Parameters 4. If available close the rotor with the rotor lid. Be sure to check all sealings before starting any aeorsol-tight applications. See the information in the rotor instruction manual. 5. The display shows: Entering Parameters Acceleration curve The Heraeus Multifuge X3 / X3R offers you a total of 9 acceleration and 10 braking curves with which samples and gradients can be centrifuged. After the centrifuge is turned on, the last running profile selected is shown. 1. Press the ACC DEC key once in order to open the acceleration profile selection menu. The display shows the message "Set acceleration". 2. Select the desired profile by entering a digit between 1-9 on the numeric pad. Curve number 1 is the slowest and curve number 9 the fastest. 3. Confirm your entry by pressing ENTER . Braking curve 1. Press the ACC DEC key twice in order to open the braking curve selection menu. The display shows the message "Set deceleration“ 2. Select the desired curve by entering a digit between 1-9 on the numeric pad. Curve number 1 is the slowest and curve number 10 the fastest. 3. Confirm your entry by pressing ENTER . Preselecting Speed / RCF 1. Press the SPEED key . The display shows the "RPM“ or the "RCF"-value depending on the display setting. Press the 4-4 Multifuge X3 / X3 R Thermo Scientific 4 Operation Entering Parameters TOGGLE key to toggle between the two modes. 2. Enter the desired value using the numeric pad. The digits show in sequential order. 3. Confirm your entry by pressing ENTER . Your entry will be automatically confirmed if you do not press any key for 5 seconds. Note If an extremely low RCF value has been selected, it will be corrected automatically if the resulting speed is less than 300 rpm. This is because 300 rpm is the lowest selectable speed. Explanation of RCF Value The relative centrifugal force is given as a multiple of the force of gravity g. It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various devices, since it is independent of the type of device. Only the centrifuging radius and the speed come into play in it: n 2 RCF = 11 , 18 × 〈 ------------- 〉 × r 1000 r = centrifuging radius in cm n = Rotational speed in rpm The maximum RCF value is related to the maximum radius of the tube opening. Remember that this value is reduced depending on the tubes and adapters used. This can be accounted for in the calculation above if required. Running time preselection 1. Press the TIME key in order to open the runtime selection menu. The display shows the message "Set Time". Enter the desired runtime in hh.mm Thermo Scientific Multifuge X3 / X3 R 4-5 4 Operation Entering Parameters 2. Press the TIME key repeatedly to switch over to mm:ss or Hold mode respectively. 3. Enter the desired runtime using the numeric pad. The digits show in sequential order. 4. Confirm your entry by pressing ENTER . Your entry will be automatically confirmed if you do not press any key for 5 seconds. While the centrifuge is in operation, two different values can be displayed. The total running time and the running time after reaching the maximum speed. Press the CHANGE key to toggle between the two display modes. Continuous operation During continuous operation, the centrifuge will continue running until you stop it manually with the STOP key . Run time mode Use the TOGGLE key to set the run time counter start. AT START When AT START is lit, the running time will be counted right from pressing the START key . AT SPEED When AT SPEED is lit, the running time will be counted once the preset speed or the preset RCF-value respectively have been reached. In this case, the selected acceleration curve has no effect on the running time. Preselecting the temperature You can preselect temperatures between -10 °C and +40 °C. While the centrifuge is in operation, two different values can be displayed. The sample temperature and the chamber temperature. Press the TOGGLE key to toggle between the two display modes. AIR When AIR is lit, the temperature inside the rotor chamber will be controlled. This setting is recommended when using the centrifuges pretemp feature. 4-6 Multifuge X3 / X3 R Thermo Scientific 4 Operation Entering Parameters SAMPLE When SAMPLE is lit, the temperature of the sample will be controlled. To set the temperature, proceed as follows: 1. Press the TEMPERATURE key in order to open the temperature selection menu. The display shows the messages "Temperature". Depending on the display mode setting, the value shown corresponds either to the sample temperature or to the inside chamber temperature. Press the TOGGLE key to toggle between the two modes. 2. Enter the desired temperature using the numeric pad. The digits show in sequential order. 3. Confirm your entry by pressing ENTER . Your entry will be automatically confirmed if you do not press any key for 5 seconds. Prewarming or precooling the centrifuge For setting the pretemp value for the centrifuge proceed as follows: 1. Hold down the TEMPERATURE key for a minimum of three seconds to open the temperature selection menu. The display shows the message "Set PreTemp". 2. Enter the desired temperature using the numeric pad. The digits show in sequential order. 3. Confirm your entry by pressing ENTER . Your entry will be automatically confirmed if you do not press any key for 5 seconds. 4. The display shows: The display shows: Thermo Scientific Multifuge X3 / X3 R 4-7 4 Operation Entering Parameters 5. Press the START key . The rotor chamber is cooled down or heated up to the preset temperature. 6. Press the STOP key . The display shows the current temperature. Bucket selection Bucket selection is only possible for swing-out rotors. The bucket code corresponds to the last four digits of the bucket catalog number. 1. Press the BUCKET key . The display shows the following message: 2. Press the BUCKET key repeatedly until the bucket being used is displayed. Your entry will be automatically confirmed if you do not press any key for 5 seconds. Bucket radius Having entered the bucket code, you may want to specify the radius. When you use adaptors, you will have to specify the radius. For appropriate values please refer to the instruction manual for the rotor. 1. Press ENTER to confirm your selection and open the radius input menu. 4-8 Multifuge X3 / X3 R Thermo Scientific 4 Operation Entering Parameters The default value is marked accordingly. You can either confirm the default value by pressing ENTER or enter a different radius. 2. Enter the radius using the numeric pad. The digits show in sequential order. 3. Press ENTER to confirm the new radius. Setting up a program Enter the program parameters. As described above, please enter braking and acceleration profile, speed/ RCF value, running time, temperatures, bucket code and bucket radius. Configuring, storing and protecting a program Programs can be stored and retrieved later by means of the assigned number. 1. Enter the program parameters as described above. 2. Press the program memory key to load any of the programs 1-5. Press the DIRECTORY key in order to load any of the programs 1-99 by means of the numeric pad. Enter the program number and confirm with ENTER key . 3. You may want to enter a passcode to protect the data. After four seconds the display shows the message "Program saved" or "Program locked". Overwriting a passcode-protected program If you wish to overwrite a passcode-protected program proceed as follows: 1. Select the program you wish to change. 2. Press the program memory key to load any of the programs 1-5. Press the DIRECTORY key in order to load any of the programs 1-99 by means of the numeric pad. Thermo Scientific Multifuge X3 / X3 R 4-9 4 Operation Centrifugation Enter the program number and confirm with ENTER key . 3. Enter the passcode. If you enter the wrong password, the following message appears: The display restores the current values. Return to step 1. Centrifugation Once the rotor has been properly installed, the main switch has been turned on and the lid has been closed, you can start centrifuging. Starting centrifuge program Press the START key on the control panel. The centrifuge accelerates to the pre-set speed with the time display active. If the speed setting is higher than the maximum permissible speed or RCF-value for the particular rotor, then the display will show the message CHECK SET SPEED. The centrifuge decelerates. Please enter a new value. You cannot open the lid as long as the centrifuge is running. Imbalance indicator If a load is imbalanced, this will be indicated at speed higher than approx. 300 rpm by the message "Imbalanced load". The run will terminate. Check the loading and start the centrifuge once again. See the information on proper loading in the rotor instruction manual. For information on troubleshooting, see section “Troubleshooting by User” on page 7-3. Stopping the centrifugation program With preset running time Usually the running time is preset and you only have to wait until the centrifuge stops automatically when the preset time limit expires. 4-10 Multifuge X3 / X3 R Thermo Scientific 4 Operation Temperature Adaptation during Standstill As soon as the speed drops to zero, the message END will appear in the display. By pressing the OPEN key , you can open the lid and remove the centrifuge material. You can also stop the centrifuging program manually at any time by pressing the STOP key . Continuous operation If you selected continuous operation (see “Continuous operation” on page 4-6), you will have to stop the centrifuge manually. Press the STOP key on the control panel. The centrifuge will be decelerated at the designated rate. The message "END" will illuminate, and after pressing the key OPEN , the lid will open and you can remove the centrifuged material. Temperature Adaptation during Standstill The temperature cannot be adapted until the rotor has been positively identified. For this, the centrifuge must have accelerated to over 500 rpm. The speed display will then show END. When the rotor is not recognized (lid closed and START key not yet pressed, speed display "0"), the centrifuge responds by ensuring that the sample cannot freeze regardless of the rotor being used. Short-term Centrifugation For short-term centrifuging, the Heraeus Multifuge X3 / X3R has a PULSE- function. By holding down the PULSE key , spinning will start and continue until the key is let go. The centrifuge accelerates and brakes at maximum power. Any rpm or RCF entered beforehand is overridden. Note The centrifuge accelerates to maximum speed, regardless of which rotor was installed. Check carefully whether you have to maintain a certain speed for your application. During the acceleration process, time is counted forwards in seconds. The reading stays displayed until the centrifuge lid is opened. Removing the Rotor To remove the rotor, proceed as follows: 1. Open the centrifuge lid. 2. Grab the rotor handle with both hands and press against the green AutoLockTM key. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to tilt the rotor while doing this. Thermo Scientific Multifuge X3 / X3 R 4-11 4 Operation Aerosol-tight Rotors Figure 4-2. Pressing AutoLockTM key Aerosol-tight Rotors When using an aerosol tight lid the rotor can only be removed with the lid closed. This is to protect you and the samples. CAUTION Rotors supplied with a lid for aerosol-tight applications come with a mandrill, which belongs to the AutoLockTM . Be sure not to place the lid onto this mandrill to prevent it from being damaged. Figure 4-3. AutoLockTM-lid for aerosol-tight rotors WARNING Mind the AutoLockTM-mandrill inside the lid. Do not touch the mandrill. Aligning the Centrifuge 1. To turn off the centrifuge put the mains switch to "0". 4-12 Multifuge X3 / X3 R Thermo Scientific System Menu Enter system menu? START Language 1.. 7: English ENTER End of run beep? No ENTER Keypad beep? No ENTER LCD powersave? No ENTER Lid passcode? No Program passcodes? No ENTER Auto lid open? No ENTER Software ID: 0547V01 ENTER Cycle count: 2368 ENTER Thermo Scientific 1 English 2 Deutsch 3 Française 4 Español 5 Italiano 6 Nederlands 7 Росский 0 no 1 yes STOP Set lid passcode ____ ENTER 0 0547V011 1 4641V05 1Software and NVRAM identification example display Multifuge X3 / X3 R 5-1 5 System Menu Description Enter system menu To enter the system menu hold down any of the keys when switching the centrifuge on. Press the ENTER key in order to navigate through the system menu. Press the STOP key to quit the system menu. Language In order to change the language in the display, press the number next to the desired language in the diagram on the numeric pad. Press ENTER key to confirm. End of run beep Press 1 (yes) on the numeric pad if the centrifuge should make beep after the run. Otherwise press 0 (no). Press ENTER key to confirm. Keypad beep Press 1 (yes) on the numeric pad if the centrifuge should make beep when pressing any key. Otherwise press 0 (no). Press ENTER key to confirm. LCD powersave Press 1 (yes) on the numeric pad if the centrifuge should enter a powdersave mode after the run. Otherwise press 0 (no). Press ENTER key to confirm. Lid passcode Press 1 (yes) on the numeric pad if the centrifuge door should be protected with a passcode. Enter passcode. If the door should not be protected with a passcode press 2 (no) on the numeric pad. Press ENTER key to confirm. Program passcodes Press 1 (yes) on the numeric pad if the centrifuge programs should be protected with a passcode. Otherwise press 0 (no). Press ENTER key to confirm. 5-2 Multifuge X3 / X3 R Thermo Scientific 5 System Menu Description Auto lid open Press 1 (yes) on the numeric pad if the centrifuge should open after the run. Otherwise press 0 (no). Press ENTER key to confirm. Software ID Here you fine the current software version. Press ENTER key to confirm. Cycle count Here you fine the current numbers of cycles. Press ENTER key to confirm. Thermo Scientific Multifuge X3 / X3 R 5-3 6 Maintenance and Care • “Cleaning Intervalls” on page 6-2 • “Cleaning” on page 6-2 • “Disinfection” on page 6-3 • “Decontamination” on page 6-4 • “Autoclaving” on page 6-5 • “Service of Thermo Fisher Scientific” on page 6-5 Thermo Scientific Multifuge X3 / X3 R 6-1 6 Maintenance and Care Cleaning Intervalls For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis. Maintenance Recommended interval Clean rotor chamber daily or when polluted Clean rotor daily or when polluted Accessories daily or when polluted Cabinet Once per month Ventilation holes Every six months CAUTION Refrain from using any other cleaning or decontamination procedure than those recommended here, if you are not entirely sure that the intended procedure is safe for the equipment. Use only approved cleansers. If in doubt, contact Thermo Fisher Scientific. Cleaning When cleaning centrifug • Use warm water with a neutral solvent. • Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or scrubbing powder. • Rinse the cavities out thoroughly. • Use a soft brush without metal bristles to remove stubborn residue. • Afterwards rinse with distilled water. • Place the rotors on a plastic grate with their cavities pointing down. • If drying boxes are used, the temperature must never exceed 50 °C, since higher temperatures could damage the material and shorten the lifetime of the parts. • Use only disinfectants with a pH of 6-8. • Dry aluminum parts off with a soft cloth. • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. 6-2 Multifuge X3 / X3 R Thermo Scientific 6 Maintenance and Care Disinfection CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Clean centrifuge and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. 5. Remove the centrifuge tubes and adaptors. 6. Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning. 7. Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50°C. • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Tread the bold of the swing out rotor with bold grease (75003786). CAUTION When cleaning, do not allow liquids, especially organic solvents, to get on the drive shaft or the bearings of the centrifuge. Organic solvents break down the grease in the motor bearing. The drive shaft could freeze up. After some applications their might be ice in the rotor chamber. Let the ice melt and drain it off. Clean the rotor chamber as described above. Disinfection Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation. WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. In case of contamination, make sure that others are not put at risk. Decontaminate the affected parts immediately. Take other precautions if need be. The rotor chamber and the rotor should be treated preferably with a neutral disinfectant. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific Multifuge X3 / X3 R 6-3 6 Maintenance and Care Decontamination Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. 5. Remove the centrifuge tubes and adaptors and dispose of them or disinfect them. 6. Treat the rotor and accessories according to the instructions for the disinfectant. Adhere strictly to the given application times. 7. Be sure the disinfectant can drain off the rotor. 8. Rinse the rotor and accessories thoroughly with water. 9. Dispose of the disinfectant according to the applicable guidelines. 10. Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50°C. • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Tread the bold of the swing out rotor with bold grease (75003786). Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation. WARNING Radioactive material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. In case of contamination, make sure that others are not put at risk. Decontaminate the affected parts immediately. Take other precautions if need be. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and water. 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. 6-4 Multifuge X3 / X3 R Thermo Scientific 6 Maintenance and Care Autoclaving 5. Remove the centrifuge tubes and adaptors and dispose of them or disinfect them. 6. Rinse the rotor first with ethanol and then with de-ionized water. • Adhere strictly to the given application times. 7. Be sure the decontamination solution can drain off the rotor. 8. Rinse the rotor and accessories thoroughly with water. 9. Dispose of the decontamination solution according to the applicable guidelines. 10. Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50°C. • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Tread the bold of the swing out rotor with bold grease (75003786). Autoclaving 1. Before autoclaving clean rotor and accessories and described above. 2. Place the rotor on a flat surface. • Rotors and adapter can beautoclaved at 121 °C. • The maximum permissible autoclave cycle is 20 minutes at 121 °C. Note No chemical additives are permitted in the steam. CAUTION Never exceed the permitted temperature and duration when autoclaving. If the rotor shows signs of corrosion or wear, it must be replaced. Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician. The service technicians check the following: • the electrical equipment • the suitability of the set-up site • the lid lock and the safety system • the rotor • the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician. Thermo Scientific Multifuge X3 / X3 R 6-5 7 Trouble Shooting • “Mechanical Emergency Door Release” on page 7-2 • “Troubleshooting by User” on page 7-3 • “When to contact Customer Service” on page 7-5 Thermo Scientific Multifuge X3 / X3 R 7-1 7 Trouble Shooting Mechanical Emergency Door Release During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop. WARNING The rotor can still be spinning at high speed. If touched, it can cause serious injuries. Always wait a few minutes until the rotor has come to a stop without braking. The brake does not work when there is no current. The braking process lasts much longer than usual. Proceed as follows: 1. Make sure the rotor has stopped (view port in the lid). WARNING Never use your hand or other tools to brake the rotor. 2. Pull out the power supply plug. 3. On the back of the housing are two black plastic plugs which you can pry out of the back plate with a screwdriver or a knife. Pull the release cord attached to it at the same time to trigger the mechanical lid release. The lid will open and the samples can be removed. Emergency Door Release Figure 7-1. Emergency Door Release Note You need to pull both levers in order to unlock both locks. 4. Push the cord back into the centrifuge and mount the plug. 7-2 Multifuge X3 / X3 R Thermo Scientific 7 Trouble Shooting Troubleshooting by User 5. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the OPEN key to have the door locks operative again. WARNING If you pull only one lever or you do not pressed the OPEN key to have the door locks operative again, the door might open while the rotor os still spinning. Troubleshooting by User If problems occur other than those listed in this table, the authorized customer service representative must be contacted. Failure message Problem with centrifuge Possible causes and cures Overtemperature The centrifuge cannot be operated. Overheating in chamber. The run does not start or the centrifuge runs Check the function of the refrigeration unit. down without being braked. Clean the air inlet for the condenser. Restart the centrifuge. If an error message appears again, inform Customer Service. Incorrect bucket ID The centrifuge cannot be operated. Bucket code is undefined for the rotor detected, check the set The run does not start or the centrifuge runs points for the given bucket code. down without being braked. Is it permitted to use the current bucket in the rotor currently mounted? Restart the centrifuge. If an error message appears again, inform Customer Service. Unapproved rotor The centrifuge cannot be operated. Rotor code is not in the rotor table. The run does not start or the centrifuge runs Is it permitted to use the rotor currently mounted in this device? down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. Rotor ID failure The centrifuge cannot be operated. The rotor could not be identified. The run does not start or the centrifuge runs Check to see if the rotor is properly installed. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. Lid blocked Centrifuge does not open Restart the centrifuge. The emergency lid release enables you to retrieve your samples. If an error message appears again, inform Customer Service. Motor overtemp. The centrifuge cannot be operated. Restart the centrifuge. The run does not start or the centrifuge runs If an error message appears again, inform Customer Service. down without being braked. Thermo Scientific Multifuge X3 / X3 R 7-3 7 Trouble Shooting Troubleshooting by User Failure message Problem with centrifuge Possible causes and cures PCB overtemp. The centrifuge cannot be operated. Restart the centrifuge. The run does not start or the centrifuge runs If an error message appears again, inform Customer Service. down without being braked. Emergency release The centrifuge cannot be operated. The lid opens while the device is running. The run does not start or the centrifuge runs Close the lid and restart the centrifuge. down without being braked. If an error message appears again, inform Customer Service. Imbalanced load The centrifuge cannot be operated. Imbalance detected. The run does not start or the centrifuge runs Check the load placed in the rotor. down without being braked. Check that the rotor cross bolts are well greased. Restart the centrifuge. If an error message appears again, inform Customer Service. Check Set Speed The centrifuge cannot be operated. The set point speed is higher than the maximum rotor speed. The run does not start or the centrifuge runs Correct the value. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. E-01 - E-12 The centrifuge cannot be operated. Error during the self-test of the centrifuge program and the The run does not start or the centrifuge runs electronics. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. E-13 The centrifuge cannot be operated. The check sum in the data memory is incorrect. The run does not start or the centrifuge runs The software corrects errors automatically. down without being braked. Check the values of the set point settings, etc. Restart the centrifuge. If an error message appears again, inform Customer Service. E-15-E-16 Temperature sensor broken / controller Malfunction in the temperature detection. defective Restart the centrifuge. If an error message appears again, inform Customer Service. E-17 Speed for rotor detection exceeded Restart the centrifuge. If an error message appears again, inform Customer Service. E-21-E-22 The centrifuge cannot be operated. The rotor could not be identified. The run does not start or the centrifuge runs Check to see if the rotor is properly installed. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. E-23 The centrifuge cannot be operated. The speed control measurement returned a different result. The run does not start or the centrifuge runs Restart the centrifuge. down without being braked. If an error message appears again, inform Customer Service. E-25-E-27 The centrifuge cannot be operated. Time has expired for the lid lock drive while opening the lid. The run does not start or the centrifuge runs Restart the centrifuge. down without being braked. If an error message appears again, inform Customer Service. 7-4 Multifuge X3 / X3 R Thermo Scientific 7 Trouble Shooting When to contact Customer Service Failure message Problem with centrifuge Possible causes and cures E-28 The centrifuge cannot be operated. Error during the self-test of the centrifuge program and the The run does not start or the centrifuge runs electronics. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. E-29 The centrifuge cannot be operated. Check wether you selected the right bucket. The run does not start. Is it easy to turn the rotor when the lid is open? Does the rotor rub against the device? Restart the centrifuge. If an error message appears again, inform Customer Service. E-30 The centrifuge cannot be operated. Restart the centrifuge. The run does not start or the centrifuge runs If an error message appears again, inform Customer Service. down without being braked. E-33 The centrifuge cannot be operated. Overpressure in the refrigeration unit. The run does not start or the centrifuge runs Clean the air inlet for the condenser. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. E-34-E-36 The centrifuge cannot be operated. Error during the self-test of the centrifuge program and the The run does not start or the centrifuge runs electronics. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. E-40 The centrifuge cannot be operated. The centrifuge accelerates too slowly. The run does not start or the centrifuge runs Is the rotor properly installed? down without being braked. Check wether you selected the right bucket. Is it easy to turn the rotor when the lid is open? Does the rotor rub against the device? Restart the centrifuge. If an error message appears again, inform Customer Service. E-41-E-74 The centrifuge cannot be operated. Error during the self-test of the centrifuge program and the The run does not start or the centrifuge runs electronics. down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. When to contact Customer Service If you need to contact customer service, please provide the order no. and the serial no. of your device. This information can be found on the back near the inlet for the power supply cable. To identify the software version, proceed as follows: 1. Hold down any of the keys and then switch on the centrifuge. You enter the system menu. 2. Press the START key . Thermo Scientific Multifuge X3 / X3 R 7-5 7 Trouble Shooting When to contact Customer Service 3. Press and hold the ENTER key , until the following message is displayed: Software ID: XXXXXXXX 4. Communicate the software version to the service technician. 7-6 Multifuge X3 / X3 R Thermo Scientific A Chemical Compatibility Chart CHEMICAL MATERIAL ALUMINUM POLYURETHANE COMPOSITE POLYCLEAR®,CLEARCRIMP®CCCLEARCRIMP® POLYALLOMER POLYCARBONATE POLYESTER, POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE BUTYRATE Fiber/Epoxy THERMOSET ALUMINUM PAINT ROTOR for COATING ACETATE Carbon PROPYLENE GLASS CHLORIDE TEFLON® RUBBER STEEL A®, ANODIC N BUNA CELLULOSE DELRIN® ETHYLENE GLASS NEOPRENE NORYL® NYLON PET1, POLYVINYL RULON SILICONE STAINLESS TITANIUM TYGON® VITON® 2-mercaptoethanol S S U - S M S - S U S S U S S - S S S S U S S S S S S Acetaldehyde S - U U - - M - U - - M U U U M M - M S U - S - U Acetone M S U U S U M S S U U S U S U U U S S U U S M M S U U Acetonitrile S S U - S M S - S S U S U M U U - S M U U S S S S U U Alconox® U U S - S S S - S S S S S S M S S S S S S S S S S S U Allyl Alcohol - U - S - S - S S M S S S - M S - S - Aluminum Chloride U U S S S S U S S S S M S S S S - S S S S S M U U S S Formic Acid (100%) - S M U - U - U - S M U U S S - U S - U S - U Ammonium Acetate S S U - S S S - S S S S S S S U - S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U - S S S S S S M S S S Ammonium Hydroxide (10%) U U S U S S M S S S S S - S U M S S S S S S S S S M S Ammonium Hydroxide (28%) U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide (conc.) U U U U S U M S - S - S U S U U S S S - M S S S S - U Ammonium Phosphate U - S - S S S S S S S S - S S M - S S S S S S M S S S Ammonium Sulfate U M S - S S U S S S S S S S S S - S S S S S S U S S U Amyl Alcohol S - M U - S S - M - S - M S S S S M - - - U - S - M Aniline S S U U S U S M S U U U U U U U - S M U U S S S S U S Sodium Hydroxide (<1%) U - M S S S - S M S S - S M M S S S S S S M S S - U Sodium Hydroxide (10%) U - M U - U - M M S S U S U U S S S S S S M S S - U Barium Salts M U S - S S S S S S S S S S S M - S S S S S S M S S S Benzene S S U U S U M U S U U S U U U M U M U U U S U U S U S Thermo Scientific Multifuge X3 / X3 R A-1 A Chemical Compatibility Chart BUTYRATE Fiber/Epoxy THERMOSET ALUMINUM PAINT ROTOR for COATING ACETATE Carbon PROPYLENE GLASS CHLORIDE TEFLON® RUBBER STEEL MATERIAL ALUMINUM ANODIC BUNA CELLULOSE POLYURETHANE COMPOSITE DELRIN® ETHYLENE GLASS NEOPRENE NORYL® NYLON PET1, POLYALLOMER POLYCARBONATE POLYESTER, POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL RULON SILICONE STAINLESS TITANIUM TYGON® VITON® A®, CHEMICAL N POLYCLEAR®,CLEARCRIMP®CCCLEARCRIMP® Benzyl Alcohol S - U U - - M M - M - S U U U U U U U - M S M - S - S Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Cesium Acetate M - S - S S S - S S S S - S S - S S S S S S M S S S Cesium Bromide M S S - S S S - S S S S S S S - S S S S S S M S S S Cesium Chloride M S S U S S S - S S S S S S S - S S S S S S M S S S Cesium Formate M S S - S S S - S S S S S S S - S S S S S S M S S S Cesium Iodide M S S - S S S - S S S S S S S - S S S S S S M S S S Cesium Sulfate M S S - S S S - S S S S S S S - S S S S S S M S S S Chloroform U U U U S S M U S U U M U M U U U M M U U S U U U M S Chromic Acid (10%) U - U U S U U - S S S U S S M U M S S U M S M U S S S Chromic Acid (50%) U - U U - S U U S M U M S S U M S - U M - S Cresol Mixture S S U - S - S U U U U U U - - U U - U S S S S U S Cyclohexane S S S - S S S U S U S S U U U M S M U M M S U M M U S Deoxycholate S S S - S S S - S S S S S S S - S S S S S S S S S S Distilled Water S S S S S S S S S S S S S S S S S S S S S S S S S S S Dextran M S S S S S S - S S S S S S S S S S S S S S S M S S S Diethyl Ether S S U U S S S U S U U S U U U U U U U U U S S S S M U Diethyl Ketone S - U U - - M - S U - S - M U U U M M - U S - S U U Diethylpyrocarbonate S S U - S S S - S S U S U S U - S S S M S S S S S S Dimethylsulfoxide S S U U S S S - S U S S U S U U - S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U - M M M U S S S S U U Ferric Chloride U U S - - M S - M - S - S - S S - - M U S - S Acetic Acid (Glacial) S S U U S S U M S U S U U U U U M S U M U S U U S - U Acetic Acid (5%) S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid (60%) S S U U S S U - S M S U U M U S M S M S M S M U S M U Ethyl Acetate M M U U S S M M S S U S U M U U - S S U U S M M S U U Ethyl Alcohol (50%) S S S S S S M S S S S S U S U S S S S S S S S M S M U Ethyl Alcohol (95%) S S S U S S M S S S S S U S U - S S S M S S S U S M U Ethylene Dichloride S - U U - S M - U U S U U U U U U U - U S U - S - S A-2 Multifuge X3 / X3 R Thermo Scientific A Chemical Compatibility Chart BUTYRATE Fiber/Epoxy THERMOSET ALUMINUM PAINT ROTOR for COATING ACETATE Carbon PROPYLENE GLASS CHLORIDE TEFLON® RUBBER STEEL MATERIAL ALUMINUM ANODIC BUNA CELLULOSE POLYURETHANE COMPOSITE DELRIN® ETHYLENE GLASS NEOPRENE NORYL® NYLON PET1, POLYALLOMER POLYCARBONATE POLYESTER, POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL RULON SILICONE STAINLESS TITANIUM TYGON® VITON® A®, CHEMICAL N POLYCLEAR®,CLEARCRIMP®CCCLEARCRIMP® Ethylene Glycol S S S S S S S S S S S S - S U S S S S S S S S M S M S Ethylene Oxide Vapor S - U - U - S U - S - S M - - S S S U S U S S S U Ficoll-Hypaque® M S S - S S S - S S S S - S S - S S S S S S S M S S S Hydrofluoric Acid (10%) U U U M - - U - - U U S - S M U S S S S M S U U U - Hydrofluoric Acid (50%) U U U U - - U - - U U U U S U U U S S M M S U U U - M Hydrochloric Acid (conc.) U U U U - U U M - U M U U M U U U - S - U S U U U - Formaldehyde (40%) M M M S S S S M S S S S M S S S U S S M S S S M S M U Glutaraldehyde S S S S - S - S S S S S S S - S S S - S S S - Glycerol M S S - S S S S S S S S S S S S - S S S S S S S S S S Guanidine Hydrochloride U U S - S S S - S S S S S S S - S S S S S S U S S S Haemo-Sol® S S S - S - S S S S S S S - S S S S S S S S S S Hexane S S S - S S S - S S U S U M U S S U S S M S U S S U S Isobutyl Alcohol - - M U - S S - U - S U S S M S S S - S S S - S - S Isopropyl Alcohol M M M U S S S S S U S S U S U M S S S S S S S M M M S Iodoacetic Acid S S M - S S S - S M S S M S S - M S S S S S M S S M M Potassium Bromide U S S - S S S - S S S S S S S S S S S - S S S M S S S Potassium Carbonate M U S S S S S - S S S S S S U S S S S S S S S S S S S Potassium Chloride U S S - S S S S S S S S S S S - S S S S S S S U S S S Potassium Hydroxide (5%) U U S S S S M - S S S S - S U S S S S S S S M U M S U Potassium Hydroxide (conc.) U U M U - - M - M S S - U M U U U S M - M U - U U - U Potassium Permanganate S S S - S S S - S S S U S S S M - S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S S M S - S S S S S S M S S S Calcium Hypochlorite M - U - S M M S - M - S - S M S - S S S M S M U S - S Kerosene S S S - S S S U S M U S U M M S - M M M S S U S S U S Sodium Chloride (10%) S - S S S S S S - S S S S S - S S S S - S S M - S Sodium Chloride (sat'd) U - S U S S S - S S S S S - S S - S - S S M - S Carbon Tetrachloride U U M S S U M U S U U S U M U S S M M S M M M M U S S Aqua Regia U - U U - - U - - U U U U U U U - S - M Solution 555 (20%) S S S - S - S S S S S S S - S S S - S S S S S S Thermo Scientific Multifuge X3 / X3 R A-3 A Chemical Compatibility Chart BUTYRATE Fiber/Epoxy THERMOSET ALUMINUM PAINT ROTOR for COATING ACETATE Carbon PROPYLENE GLASS CHLORIDE TEFLON® RUBBER STEEL MATERIAL ALUMINUM ANODIC BUNA CELLULOSE POLYURETHANE COMPOSITE DELRIN® ETHYLENE GLASS NEOPRENE NORYL® NYLON PET1, POLYALLOMER POLYCARBONATE POLYESTER, POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL RULON SILICONE STAINLESS TITANIUM TYGON® VITON® A®, CHEMICAL N POLYCLEAR®,CLEARCRIMP®CCCLEARCRIMP® Magnesium Chloride M S S - S S S S S S S S S S S S S S S S S S S M S S S Mercaptoacetic Acid U S U - S M S - S M S U U U U - S U U S M S U S S S S Methyl Alcohol S S S U S S M S S S S S U S U M S S S S S S S M S M U Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U Methyl Ethyl Ketone S S U U S S M S S U U S U S U U U S S U U S S S S U U Metrizamide® M S S - S S S - S S S S - S S - S S S S S S M S S S Lactic Acid (100%) - S - - M S U - S S S M S S - M S M S S - S Lactic Acid (20%) - S S - - M S M - S S S S S S S M S M S S - S N-Butyl Alcohol S - S U - S - S M - U S M S S S S M M S M - S - S N-Butyl Phthalate S S U - S S S - S U U S U U U M - U U S U S M M S U S N, N-Dimethylformamide S S S U S M S - S S U S U S U U - S S U U S M S S S U Sodium Borate M S S S S S S S S S S U S S S S - S S S S S S M S S S Sodium Bromide U S S - S S S - S S S S S S S S - S S S S S S M S S S Sodium Carbonate (2%) M U S S S S S S S S S S S S U S S S S S S S S S S S S Sodium Dodecyl Sulfate S S S - S S S - S S S S S S S - S S S S S S S S S S S Sodium Hypochlorite (5%) U U M S S M U S S M S S S M S S S S M S S S M U S M S Sodium Iodide M S S - S S S - S S S S S S S - S S S S S S M S S S Sodium Nitrate S S S - S S S S S S S S S S S S - S S S S S U S S S S Sodium Sulfate U S S - S S S S S S S S S S S S S S S S S S S M S S S Sodium Sulfide S - S S - S - S S S U U - S - S S M - S Sodium Sulfite S S S - S S S S M S S S S S S M - S S S S S S S S S S Nickel Salts U S S S S S - S S S - S S S S - S S S S S S M S S S Oils (Petroleum) S S S - S U S S S S U U M S M U U S S S U S S S S Oils (Other) S - S - S M S S S S U S S S S U S S S S - S S M S Oleic Acid S - U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S Perchloric Acid (10%) U - U - S U U - S M M - - M U M S M M - M S U - S - S Perchloric Acid (70%) U U U - - U U - S U M U U M U U U M M U M S U U S U S Phenol (5%) U S U - S M M - S U M U U S U M S M S U U S U M M M S A-4 Multifuge X3 / X3 R Thermo Scientific A Chemical Compatibility Chart BUTYRATE Fiber/Epoxy THERMOSET ALUMINUM PAINT ROTOR for COATING ACETATE Carbon PROPYLENE GLASS CHLORIDE TEFLON® RUBBER STEEL MATERIAL ALUMINUM ANODIC BUNA CELLULOSE POLYURETHANE COMPOSITE DELRIN® ETHYLENE GLASS NEOPRENE NORYL® NYLON PET1, POLYALLOMER POLYCARBONATE POLYESTER, POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL RULON SILICONE STAINLESS TITANIUM TYGON® VITON® A®, CHEMICAL N POLYCLEAR®,CLEARCRIMP®CCCLEARCRIMP® Phenol (50%) U S U - S U M - S U M U U U U U S U M U U S U U U M S Phosphoric Acid (10%) U U M S S S U S S S S U - S S S S S S S S S U M U S S Phosphoric Acid (conc.) U U M M - - U S - M S U U M M S S S M S M S U M U - S Physiologic Media (Serum, Urine) M S S S - S - S S S S S S S S S S S S S S S S S S S Picric Acid S S U - S M S S S M S U S S S U S S S S U S U M S M S Pyridine (50%) U S U U S U U - U S S U U M U U - U S M U S S U U U U Rubidium Bromide M S S - S S S - S S S S S S S - S S S S S S M S S S Rubidium Chloride M S S - S S S - S S S S S S S - S S S S S S M S S S Sucrose M S S - S S S S S S S S S S S S S S S S S S S S S S S Sucrose, Alkaline M S S - S S S - S S S S S S U S S S S S S S S M S S S Sulfosalicylic Acid U U S S S S S - S S S U S S S - S S S - S S S U S S S Nitric Acid (10%) U S U S S U U - S U S U - S S S S S S S S S M S S S S Nitric Acid (50%) U S U M S U U - S U S U U M M U M M M S S S U S S M S Nitric Acid (95%) U - U U - U U - - U U U U M U U U U M U U S U S S - S Hydrochloric Acid (10%) U U M S S S U - S S S U U S U S S S S S S S S U M S S Hydrochloric Acid (50%) U U U U S U U - S M S U U M U U S S S S M S M U U M M Sulfuric Acid (10%) M U U S S U U - S S M U S S S S S S S S S S U U U S S Sulfuric Acid (50%) M U U U S U U - S S M U U S U U M S S S S S U U U M S Sulfuric Acid (conc.) M U U U - U U M - - M U U S U U U M S U M S U U U - S Stearic Acid S - S - S M S S S S - S S S S S S S S S M M S S S Tetrahydrofuran S S U U S U U M S U U S U U U - M U U U U S U S S U U Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M Trichloroacetic Acid U U U - S S U M S U S U U S M - M S S U U S U U U M U Trichloroethane S - U - - M U - U - S U U U U U U U U U S U - S - S Trichloroethylene - - U U - - U - S U U U U U U U U U S U - U - S Trisodium Phosphate - S - - M - - S - S S S - S - S - S Tris Buffer (neutral pH) U S S S S S S - S S S S S S S S S S S S S S S S S S S Triton X-100® S S S - S S S - S S S S S S S S S S S S S S S S S S S Urea S - U S S S S - S S S M S S S S - S S S M S - S Thermo Scientific Multifuge X3 / X3 R A-5 A Chemical Compatibility Chart BUTYRATE Fiber/Epoxy THERMOSET ALUMINUM PAINT ROTOR for COATING ACETATE Carbon PROPYLENE GLASS CHLORIDE TEFLON® RUBBER STEEL MATERIAL ALUMINUM ANODIC BUNA CELLULOSE POLYURETHANE COMPOSITE DELRIN® ETHYLENE GLASS NEOPRENE NORYL® NYLON PET1, POLYALLOMER POLYCARBONATE POLYESTER, POLYTHERMIDE POLYRTHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL RULON SILICONE STAINLESS TITANIUM TYGON® VITON® A®, CHEMICAL N POLYCLEAR®,CLEARCRIMP®CCCLEARCRIMP® Hydrogen Peroxide (10%) U U M S S U U - S S S U S S S M U S S S S S S M S U S Hydrogen Peroxide (3%) S M S S S - S - S S S S S S S S M S S S S S S S S S S Xylene S S U S S S M U S U U U U U U M U M U U U S U M S U S Zinc Chloride U U S S S S U S S S S S S S S S S S S S S S S U S S S Zinc Sulfate U S S - S S S S S S S S S S S S S S S S S S S S S S S Citric Acid (10%) M S S M S S M S S S S S S S S S M S S S S S S S S S S 1Polyethyleneterephthalate Key S Satisfactory M Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc. Suggest testing under actual conditions of use. U Unsatisfactory, not recommended. -- Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. No organized chemical resistance data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting sample lots. A-6 Multifuge X3 / X3 R Thermo Scientific B Contact Information United States 866-9-THERMO +1 866 984 3766 Canada +1 866 984 3766 Austria +43 1 801 400 Belgium +32 2 482 30 30 Germany 08001 536 376 +49 6184 90 6940 France +33 2 2803 2180 +33 2 2803 2000 Italy +39 02 02 95059 341 Netherlands +31 76 571 4440 Nordic / Baltic Countries +35 89 329 100 Russia +7 (812) 703 42 15 Thermo Scientific Multifuge X3 / X3 R B-1 B Contact Information Spain +34 932 23 09 18 Portugal +34 932 23 09 18 Switzerland +41 44 454 12 12 UK / Ireland +44 870 609 9203 China +86 21 6865 4588 +86 10 8419 3588 India +91 22 6716 2200 Japan +81 45 453 9220 Other Asian Countries +852 2885 4613 Latin America +1 866 984 3766 Other Countries +49 6184 90 6940 +33 2 2803 2180 Other Countries +49 6184 90 6940 +33 2 2803 2180 B-2 Multifuge X3 / X3 R Thermo Scientific Index A Acceleration curve ...........................................4-4 Accident Prevention .......................................... iv Aerosol-tight Rotors ......................................4-12 Aligning the Centrifuge ...................................2-3 Autoclaving .....................................................6-5 B Braking curve ..................................................4-4 Bucket radius ..................................................4-8 Bucket selection ..............................................4-8 C Care ................................................................6-1 Centrifugation ...............................................4-10 Cleaning ..........................................................6-2 Cleaning Intervalls ..........................................6-2 Close Lid .........................................................4-2 Continuous Operation ...........................4-6, 4-11 Control Panel ..................................................3-2 D Decontamination ............................................6-4 Disinfection ....................................................6-3 E Emergency Door Release .................................7-2 Entering Parameters ........................................4-4 Explanation of RCF Value ..............................4-5 F Failure message ...............................................7-3 Features ...........................................................1-5 I Imbalance indicator .......................................4-10 Intended Use ...................................................... v Thermo Scientific I K Keys ................................................................3-2 Kundendienst ...........................................6-5, 7-5 L Lid Opening ....................................................4-2 Location ..........................................................2-2 M Mains Connection ...........................................2-3 Mains Supply ...........................................1-5, 2-4 Maintenance ...................................................6-1 O Operation ........................................................4-1 Overwriting a passcode-protected program ......4-9 Overwriting a program ....................................4-9 P Passcode ..........................................................4-9 Precautions ........................................................ iv Preface ...............................................................iii Preselecting RCF .............................................4-4 Preselecting Speed ...........................................4-4 Preselecting the temperature ............................4-6 Preset Running Time ....................................4-10 Prewarming or Precooling ...............................4-7 Prewarming or precooling the centrifuge .........4-7 R Removing the Rotor ......................................4-11 Rotor Installation ............................................4-2 Rotor Selection ................................................1-6 Running time preselection ...............................4-5 S Safety features .................................................1-2 Sorvall Legend XT I-1 Index: Safety zone ......................................................2-2 Select Program ................................................4-9 Short-term Centrifugation ............................4-11 Starting centrifuge program ...........................4-10 Stopping the centrifugation program .............4-10 Storage ............................................................2-4 Switch on Centrifuge ......................................4-2 T Technical Data ................................................1-3 Temperature Adaptation ...............................4-11 Transporting the Centrifuge ............................2-2 Trouble Shooting ............................................7-1 I-2 Sorvall Legend XT

Storage

on page 2-4 Thermo Scientific Multifuge X3 / X3 R 2-1 2 Before use Before Setting up 1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately if any damage is discovered. 2. Remove the packaging. 3. Check the order for completeness (see “Scope of Supply” on page iii). If the order is incomplete, please contact Thermo Fisher Scientific. Transporting the Centrifuge • Due to its weight (see “Technical Data” on page 1-3), the centrifuge should be carried by several people. • Always lift the centrifuge at both sides. Figure 2-1. Lifting the centrifuge at both sides. • The centrifuge can be damaged by impacts. • Transport the centrifuge upright and if at all possible in its packaging. WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front or the back panel. Always remove the rotor before moving the centrifuge. Location The centrifuge should only be operated indoors. The set-up location must fulfil the following requirements: • A safety zone of at least 30 cm must be maintained around the centrifuge. People and hazardous substances must be kept out of the safety zone while centrifuging. • The supporting structure must be stable and free of resonance, for example a level laboratory bench. • The supporting structure must be suitable for horizontal setup of the centrifuge. 2-2 Multifuge X3 / X3 R Thermo Scientific 2 Before use Aligning the Centrifuge • The centrifuge should not be exposed to heat and strong sunlight. WARNING UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight. • The set-up location must be well-ventilated at all times. Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location. The supporting structure must be suitable for horizontal setup of the centrifuge. CAUTION If the centrifuge isn’t level, imbalances can occur and the centrifuge can be damaged. Do not place anything under the feet to level the centrifuge. Mains Connection Main Power Switch Mains plug Figure 2-2. Mains Connection 1. Turn off the power supply switch on the back (press "0"). 2. Plug the centrifuge into grounded electrical sockets only. 3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Thermo Scientific Multifuge X3 / X3 R 2-3 2 Before use • Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. 2-4 Multifuge X3 / X3 R Thermo Scientific Control Panel