ApoBrdU DNA Fragmentation Assay

Hazard statements (CLP)

H225, H301+H331, H315, H315+H320, H319, H335, H413

Code

Statements

H225

Highly Flammable liquid and vapour

SECTION 2: Hazards identification

NFPA Rating Component Description Volume Safety Information Positive Control Cells Clear solution in 70% ethanol 5 ml See below Negative Control Cells Clear solution in 70% ethanol 5 ml See below Wash Buffer Proprietary Buffer 120 ml No hazards Reaction Buffer Proprietary Buffer (contains C2H7AsO2) 0.6 ml See below TdT Enzymes Lyophilized 45 µl No hazards Br-dUTP Clear solution (contains 5-Bromo-2’-deoxyuridine 5’- 0.48 ml No hazards triphosphate) Rinse Buffer Proprietary Buffer 120 ml No hazards Anti-BrdU-FITC Antibody Fluorescein labeled antibody in solution 0.3 ml No hazards PI/RNase Staining Buffer Proprietary Buffer 30 ml No hazards Ethanol: Emergency Overview

2.3 Other hazards

Highly flammable liquid, Target organ effect, Irritant, Carcinogen Target Organs: Nerves, Liver, Heart Carcinogen, Target organ effect, Toxic by inhalation, Toxic by ingestion, Irritant, Teratogen Target Organs: Kidney, Gastrointestinal tract, Skin, Heart, Brain, Bone marrow, Nerves, Liver

2.1 Classification of the substance or mixture

Flammable liquids (Category 2) Skin irritation (Category 2) Eye irritation (Category 2B) Specific target organ toxicity – single exposure (Category 3) Acute toxicity, Oral (Category 3) Acute toxicity, Inhalation (Category 3) Skin irritation (Category 2) Eye irritation (Category 2A) Specific target organ toxicity – single exposure (Category 3) Chronic aquatic toxicity (Category 4)

Signal word

Danger warning

Hazard statements

H225 Highly flammable liquid and vapor H315+H320 Causes skin and eye irritation. H335 May cause respiratory irritation. H301+H331 Toxic if swallowed inhaled or in contact with skin H315 Causes skin irritation. H319 Causes serious eye irritation. H335 May cause respiratory irritation. H413 May cause long lasting harmful effects to aquatic life.

Precautionary statements

P210 Keep away from heat/sparks/open flames/hot surfaces. No smoking P261 Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P261 Avoid breathing dust/fume/gas/mist/vapors/spray P301+P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P311 Call a POISON CENTER or doctor/physician.

Health hazards

2 Flammability: 3 2 Fire: 3 Reactivity Hazard: 0 2 Flammability: 0 2 Fire: 0 Reactivity Hazard: 0

Physical hazards

0 0

Potential health effects

Inhalation: May be harmful if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: May be harmful if swallowed. Cacodylic acid: Emergency Overview Inhalation: Toxic if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: Toxic if swallowed.

Medical Conditions Generally Aggravated by Exposure

Cacodylic acid may be partially reduced to inorganic arsenic in the body. Exposure to arsenic compounds can cause burning, dry nose, dry mouth, muscle cramps/spasms. May cause irritation of the gastrointestinal tract vomiting, nausea, diarrhea, shock, even death.