CaCl2

Hazard statements (CLP)

H301, H311, H315, H319, H331, H335, H373, H412

Code

Statements

H301

Toxic if swallowed

2.1 Classification of the substance or mixture

ELINCS n° w/w Not classified as dangerous Not classified as hazardous Labeling symbols: ELINCS n° w/w

67/548/EEC

1272/2008/EC Tris HCl 214-684-5 1185-53-1 < 0.5 % Xi R36/37/38 Eye Irrit. 2, H319 Skin Irrit. 2, H315 STOT SE 3, H335 Tris 201-064-4 77-86-1 < 0.05% Xi R36/38 Eye Irrit. 2, H319 Skin Irrit. 2, H315 For exposure limits see ch. 8, for phrases R and hazard statements text see ch. 16 44.. FFIIRRSSTT AAIID D M MEEAASSUURREESS Ingestion: If swallowed rinse mouth with plenty of water provided person is conscious. Get medical advice if adverse symptoms appear. Inhalation exposure: If inhaled, move person to fresh air. Get medical advice if adverse symptoms appear. Contact with skin: Remove contaminated clothes and shoes. Wash affected area with soap or mild detergent and plenty of water. Get medical advice if adverse symptoms appear. Contact with eyes: Wash immediately with plenty of water or normal saline. Keep eyelid open with the finger. Get medical advice if adverse symptoms appear. 55.. FFIIRREE--FFIIGGH HTTIIN NGG M MEEAASSUURREESS Suitable extinguishing means: Water spray or regular foam, CO2, dry powder. Mean of extinguishing NOT to be Not known. used: Known 1272/2008/EC p-Nitroaniline (*) 202-810-1 100-01-6 < 0.0001 % T, R23/24/25 Acute Tox. 3 (*), H331 R33 Acute Tox. 3 (*), H311 R52/53 Acute Tox. 3 (*), H301 STOT RE 2 (*), H373 Aquatic Chronic 3, H412 Dipotassium [[N,N'-ethylenebis[N- 239-803-8 15708-48-2 < 6 % Xi, R36/37/38 Eye Irrit. 2, H319 (carboxymethyl)glycinato]](4-)- Skin Irrit.2, H315 N,N',O,O',ON,ON']magnesate(2-) STOT SE 3, H335 For exposure limits see ch. 8, for phrases R and hazard statements text see ch. 16 44.. FFIIRRSSTT AAIID D M MEEAASSUURREESS Ingestion: If swallowed rinse mouth with plenty of water provided person is conscious. Get medical advice if adverse symptoms appear. Inhalation exposure: If inhaled, move person to fresh air. Get medical advice if adverse symptoms appear. Contact with skin: Remove contaminated clothes and shoes. Wash affected area with soap or mild detergent and plenty of water. Get medical advice if adverse symptoms appear. Contact with eyes: Wash immediately with plenty of water or normal saline. Keep eyelid open with the finger. Get medical advice if adverse symptoms appear. 55.. FFIIRREE--FFIIGGH HTTIIN NGG M MEEAASSUURREESS Suitable extinguishing means: Water spray or regular foam, CO2, dry powder. Mean of extinguishing NOT to be Not known. used: Known hazards caused by Thermal decomposition or combustion may generate toxic and hazardous fumes (COx, NOx, HCl). combustion: Equipment for self-protection: Self-contained breathing apparatus, flame and chemical resistant clothing, boots and gloves. (fire fighters): 66.. AACCCCIIDDEEN NTTAALL RREELLEEAASSEE MEEAASSUURREESS M Personal precautions: Suitable protective clothing, rubber or polythene gloves, rubber shoes, safety glasses. Environmental precautions: Do not let the product enter drainage system, surface and ground-water or soil. Contact local authorities in case of environmental release. Do not empty into drains. Cleaning procedure to recover Soak up with inert absorbent material, and clean with plenty of water. Send to the storage waiting for spilled material: disposal procedures. 77.. H HAAN ND DLLIIN NGG AAN ND D SSTTO ORRAAGGEE 7.1 Handling Handling procedures: Wear suitable protective clothing, gloves, eye protection. When use do not eat, drink or smoke. Provide sufficient ventilation in all work areas. Work/Hygienic practices: Wash hands with soap and water after use. 7.2

SECTION 2: Hazards identification

caused by Thermal decomposition or combustion may generate toxic and hazardous fumes (COx, NOx, HCl, combustion: Na2O). Equipment for self-protection: Self-contained breathing apparatus, flame and chemical resistant clothing, boots and gloves. (fire fighters): 66.. AACCCCIIDDEEN NTTAALL RREELLEEAASSEE M MEEAASSUURREESS Personal precautions: Suitable protective clothing, rubber or polythene gloves, rubber shoes, safety glasses. Environmental precautions: Do not let the product enter drainage system, surface and ground-water or soil. Contact local authorities in case of environmental release. Do not empty into drains. Cleaning procedure to recover Soak up with inert absorbent material, and clean with plenty of water. Send to the storage waiting for spilled material: disposal procedures. 77.. H HAAN ND DLLIIN NGG AAN ND D SSTTO ORRAAGGEE 7.1 Handling Handling procedures: Wear suitable protective clothing, gloves, eye protection. When use do not eat, drink or smoke. Provide sufficient ventilation in all work areas. Work/Hygienic practices: Wash hands with soap and water after use. 7.2

Storage

Room ventilation: Well ventilated workplace. Special precautions: Avoid environmental release. (see also Section 8) Recommended temperature: Store at 2 – 8 °C. Humidity, light and other Avoid light exposure and keep away from heat sources and non compatible materials. environmental factors: Containers: Keep containers tightly closed and labelled with the name of the product. Other storage precautions: Keep away from food and drinks. 88.. EEXXPPO OSSUURREE CCO ON NTTRRO OLLSS//PPEERRSSO ON NAALL PPRRO OTTEECCTTIIO ON N 8.1 Exposure limit values TLV/TWA: not available LV EU: not available TLV /STEL: not available 8.2 Exposure Controls Respiratory protection: Respiratory protection is not required. Where risk assessment shows air-purifying respirators are appropriate, use masks with approved filter. Skin protection: Protective clothing, rubber or polythene gloves. Eye protection: Safety glasses. Hand protection: Rubber or polythene gloves. Other protective systems: Personal protective equipment (PPE) useful for reducing individual exposure. Environmental protection: Avoid any release into the environment. 99.. PPH HYYSSIICCAALL AAN NDD CCH HEEM MIICCAALL PPRRO OPPEERRTTIIEESS 9.1 General information Room ventilation: Well ventilated workplace. Special precautions: Avoid environmental release. (see also Section 8) Recommended temperature: Store at 2 - 8 °C. Humidity, light and other Avoid light exposure and keep away from heat sources and non compatible materials. environmental factors: Containers: Keep containers tightly closed and labelled with the name of the product. Other storage precautions: Keep away from food and drinks. 88.. EEXXPPO OSSUURREE CCO ON NTTRRO OLLSS//PPEERRSSO ON NAALL PPRRO OTTEECCTTIIO ON N 8.1 Exposure limit values TLV/TWA (ACGIH): 3 mg/m3 (skin), 2007, related to EU OEL: not available p-nitroaniline (1) OSHA PEL : 8hr-TWA 6 mg/m3 (1 ppm) (skin), 1994, OEL France: VME 3 mg/m3, Skin, Feb 2006, related to p-nitroaniline (1) related to p-nitroaniline (1) TLV /STEL: not available OEL Belgium: TWA 3 mg/m3, Skin, Mar 2002, related to p-nitroaniline (1) NIOSH REL: 10hr-TWA 3 mg/m3 (skin), 1992, related to (2) p-nitroaniline IDLH: 300 mg/m3 related to p-nitroaniline (2) 8.2 Exposure Controls Respiratory protection: Respiratory protection is not required. Where risk assessment shows air-purifying respirators are appropriate, use masks with approved filter. Skin protection: Protective clothing, rubber or polythene gloves. Eye protection: Safety glasses. Hand protection: Rubber or polythene gloves. Other protective systems: Personal protective equipment (PPE) useful for reducing individual exposure. Environmental protection: Avoid any release into the environment. 99.. PPH HYYSSIICCAALL AAN ND D CCH HEEM MIICCAALL PPRRO OPPEERRTTIIEESS 9.1 General information

Appearance/Odor

Solid not available Color: White to off- white 9.2 Important health, safety and environmental information Value Related to pH: not available Flammability: not available Explosive properties: not available Oxidizing properties: not available Density: not available Solubility: not available Water Solubility: miscible Mixture 9.3 Solid not available Color: White to off white 9.2 Important health, safety and environmental information Value Related to pH: not available Flammability: not available Explosive properties: not available Oxidizing properties: not available Density: not available Solubility: not available Water Solubility: miscible Mixture 9.3

Further information

Melting point/range: not available 1100.. SSTTAABBIILLIITTYY AAN ND D RREEAACCTTIIVVIITTYY Stability: The product is stable until the expiration date shown on the box and on the labels when stored at 2 – 8 °C. 10.1 Conditions to avoid: Keep out from heat ,water, humidity and light. 10.2 Materials to avoid: Strong oxidizing agents. 10.3 Hazardous decomposition Thermal decomposition or combustion may include toxic and hazardous fumes of COx, NOx, HCl, products: Na2O. 1111.. TTO OXXIICCO OLLO OGGIICCAALL IIN NFFO ORRM MAATTIIO ON N 11.1 Toxicokinetic effects (ADME) Absorption: not available Distribution: not available Metabolism: not available Excretion: not available 11.2 Acute toxicity Value m.u. Effects Related to Oral: LD50 (rat) = 5,900 mg/Kg (1) Tris Dermal: LD50 (rat) = not available mg/Kg Inhalation: LC50 (rat) = not available mg/m3 Other data: not available 11.3 Irritation Skin: not available Eye: not available Inhalation: not available 11.4 Sensitization: Skin sensitization: not available Sensitization by inhalation: not available 11.5 Prolonged exposure not available toxicity: 11.6 CMR effects Related to Mutagenicity: Ames test: not available Mouse lymphoma: not available Chromosomal aberration: not available Micronucleus test: not available Teratogenesis: not available Carcinogenesis: not available 1122.. EECCO OLLO OGGIICCAALL IIN NFFO ORRM MAATTIIO ON N 12.1 Ecotoxicology Value m.u. Related to Acute toxicity with fish: LC50 = not available mg/l/96 hours Acute toxicity with Daphnia EC50 = not available mg/l/48 hours Magna: Acute toxicity with algae: EC50 = not available mg/l/72 hours 12.2 Mobility: not available 12.3 Persistency and not available degradability: 12.4 Bioaccumulation potential: not available 12.5 Evaluation PBT result: not available 12.6 Other toxic effects: not available 1133.. DDIISSPPO OSSAALL CCO ON NSSIID DEERRAATTIIO ON N National laws on disposal must be considered, local and UE requirements for wastes recycling must be respected. Used waste product, surplus product or spillage products shall be disposed of in accordance with national, state and local laws. 1144.. TTRRAAN NSSPPO ORRTT IIN NFFO ORRM MAATTIIO ON N Not classified as dangerous for transport in accordance with ADR/RID, IMDG, IATA and DOT regulations. 1155.. RREEGGUULLAATTO ORRYY IIN NFFO ORRM MAATTIIO ON N Regulatory information on labeling according to 67/548/EEC, to 1999/45/EEC Directive and to 1272/2008 Regulation (EC)(European reinforcement of GHS), and according to their following amendments/atp. According to 67/548/EEC and According to 1999/45/EEC Directives 1272/2008/EC Regulation Melting point/range: not available 1100.. SSTTAABBIILLIITTYY AAN ND D RREEAACCTTIIVVIITTYY Stability: The product is stable until the expiration date shown on the box and on the labels when stored at 2 -8 °C. 10.1 Conditions to avoid: Keep out from heat, water, humidity and light. 10.2 Materials to avoid: Strong oxidising agents. 10.3 Hazardous decomposition Thermal decomposition or combustion may include toxic and hazardous fumes of COx, NOx, HCl. products: 1111.. TTO OXXIICCO OLLO OGGIICCAALL IIN NFFO ORRM MAATTIIO ON N 11.1 Toxicokinetic effects (ADME) Absorption: p-Nitroaniline can be absorbed by inhalation of vapors or dust and through the skin. (3) Distribution: not available Metabolism: not available Excretion: p-Nitroaniline is eliminated within 3 days. Its metabolites are excreted principally in the urine and to a lesser extent in the faeces. (3) 11.2 Acute toxicity Value m.u. Effects Related to Oral: LD50 (rat) = 750 mg/Kg (3) p-Nitroaniline Dermal: LD50 (rat) > 2,500 mg/Kg (3) p-Nitroaniline LD50 (guinea pig) > 500 mg/Kg (1) p-Nitroaniline Inhalation: LC50(rat) = not available mg/m3 Other data: TDLO inhalation (rat) = 80 mg/m3/6h/4 week (1) p-Nitroaniline intermittent 11.3 Irritation Skin: Solid form of p-Nitroaniline is irritating to skin and eyes. (3) Eye: p-Nitroaniline is mildly irritating to eyes and may cause corneal damage. (3) Inhalation: p-Nitroaniline dust is irritating nose and throat. (3) 11.4 Sensitization: Skin sensitization: p-Nitroaniline showed no sensitizing power on the guinea pig. (3) Sensitization by inhalation: not available 11.5 Prolonged exposure The repeated exposure to p-Nitroaniline can cause methemoglobinemia and hemolysis, anemia and toxicity: jaundice, liver damage. (3) 11.6 CMR effects Related to Mutagenicity: Ames test: Mutagenic in some Salmonella strains. (3) p-Nitroaniline Mouse lymphoma: not available Chromosomal aberration: not available Micronucleus test: not available Teratogenesis: Reproductive toxicity studies performed with p-Nitroaniline on rats showed: at low doses fetotoxicity associated with weight gain of the spleen, and at high doses embryotoxicity associated with maternal toxicity. (3) Carcinogenesis: In a two-year study, the administration of p-Nitroaniline to mice by gavage showed inconclusive evidence of carcinogenic activity in male mice, based to increased incidence of hemangiomas of the liver and haemangiosarcomas or haemangiosarcomas (combined) in other locations. In female mice is not observed evidence of carcinogenic activity. (3) 1122.. EECCO OLLO OGGIICCAALL IIN NFFO ORRM MAATTIIO ON N 12.1 Ecotoxicology Value m.u. Related to Acute toxicity with fish: LC50 fish = 87.6 mg/l/96 hours (3) p-Nitroaniline Acute toxicity with Daphnia EC50 crustaceans = 24 mg/l/48 hours (3) p-Nitroaniline Magna: Acute toxicity with algae: EC50 = not available mg/l/72 hours 12.2 Mobility: If released to soil, p-Nitroaniline is expected to have high mobility based upon Koc values of 54-87. If released to air, p-nitroaniline will exist in both the vapor and particulate phases in the atmosphere. If released into water, p-nitroaniline is not expected to adsorb to suspended solids and sediment based upon the Koc values. (4) 12.3 Persistency and Volatilization of p-Nitroaniline from water and moist soil surfaces, and its hydrolysis are not expected to be degradability: an important fate process. (4) 12.4 Bioaccumulation potential: In a 6-week bioconcentration test, the BCF of p-nitroaniline was 2.9-3.6 at a concentration of 0.5 mg/L and <10 at a concentration of 0.05 mg/L. In a bioconcentration study using zebrafish and a p-nitroaniline concentration of 0.21 µmol/L, the BCF was measured to be 4.4. These experimental BCF values suggest bioconcentration in aquatic organisms is low. (4) 12.5 Evaluation PBT result: not available 12.6 Other toxic effects: not available 1133.. DDIISSPPO OSSAALL CCO ON NSSIID DEERRAATTIIO ON N National laws on disposal must be considered, local and UE requirements for wastes recycling must be respected. Used waste product, surplus product or spillage products shall be disposed of in accordance with national, state and local laws. 1144.. TTRRAAN NSSPPO ORRTT IIN NFFO ORRM MAATTIIO ON N Not classified for transport in accordance with ADR/RID, IMDG, IATA and DOT regulations. 1155.. RREEGGUULLAATTO ORRYY IIN NFFO ORRM MAATTIIO ON N Regulatory information on labeling according to 67/548/EEC, to 1999/45/EEC Directive and to 1272/2008 Regulation (EC)(European reinforcement of GHS), and according to their following amendments/atp. According to 67/548/EEC and According to 1999/45/EEC Directives 1272/2008/EC Regulation Classification: Not classified as dangerous Not classified as hazardous Labeling symbols: (signal word) none Labeling risk phrases: (hazard statements) none Labeling safety phrases: (precautionary statements) none Other labeling details:  1.5% of this mixture consist of ingredients of unknown toxicity for human health and aquatic environment. Safety precautions: Wear suitable protective clothing, gloves and eye/face protection. Authorization: no Restriction: no 1166.. O OTTH HEERR IIN NFFO ORRM MAATTIIO ON N Phrases R: R23/24/25: Toxic by inhalation, in contact with skin and if swallowed. R33: Danger of cumulative effects. R52/53: Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. R36/37/38: Irritating to eyes, respiratory system and skin. Hazard Statements: H331: Toxic if inhaled. H311: Toxic in contact with skin. H301: Toxic if swallowed. H373: May cause damage to organs through prolonged or repeated exposure. H412: Harmful to aquatic life with long lasting effects. H319: Causes serious eye irritation. H315: Causes skin irritation. H335: May cause respiratory irritation. The contained information in this MSDS are in accordance with Annex II of Regulation no.1907/2006 (REACh) and in accordance with ANSI “Standard for Hazardous Industrial Chemicals - Material Safety Data Sheets – Preparation” (ANSI Z400.1-2004) as recommended by US OSHA. Bibliographic references: (1) RTECS:BY7000000 The Registry of Toxic Effects of Chemical Substances, p-Nitroaniline (2) OSHA/EPA Occupational Chemical Database - Full Report, P-NITROANILINE (3) http://www.salute.gov.it/sicurezzaChimica, MSDS for p-nitroaniline, Code RE 1623 (4) Hazardous Substances Data Bank (HSDB), p-Nitroaniline, HSN: 1156 (*) Classification in Annex I of Dir 67/548/EEC and in Annex VI of the 1272/2008/EC Regulation 1.1 Identification of the mixture Product Name: Buffer, stock solution Product Number: 000654087L 1.2 Use of the mixture: For in vitro diagnostic use. 1.3 Company identification: MANUFACTURER: DISTRIBUTOR EU: Instrumentation Laboratory Co. Via Roma, 103 180 Hartwell Road, 20040 Cavenago Brianza (Italy) Bedford, MA 01730-2443 (USA) Tel. +1 800 678 0710 DISTRIBUTOR US/CANADA: Fax +1 781 863 9928 DiaPharma Group, Inc. 8948 Beckett Rd. West Chester, OH 45069 (US) E-mail address of the competent person: infosds@mail.ilww.it 1.4 Emergency phone: +44 (0)3700 492 795 +1 215 207 0061 (USA and Canada) 22.. H HAAZZAARRD DSS IID DEEN NTTIIFFIICCAATTIIO ON N 2.1 Mixture classification (see also ch. 15) Classified: not dangerous according to 67/548/EEC and 1999/45/EEC Directives Classified: not hazardous according to 1272/2008/EC Regulation 2.2 Potential health and environmental effects

Signal word

none Labeling risk phrases: none

Hazard statements

Labeling safety phrases: H319: Causes serious eye irritation. H315: Causes skin irritation. H335: May cause respiratory irritation. The contained information in this MSDS are in accordance with Annex II of Regulation no.1907/2006 (REACh) and in accordance with ANSI “Standard for Hazardous Industrial Chemicals - Material Safety Data Sheets – Preparation” (ANSI Z400.1-2004) as recommended by US OSHA. Bibliographic references: (1) ChemIDplus Lite, Tromethamine, full record 1.1 Identification of the mixture Product Name: S-2765 15.4 mg + I-2581 Product Number: 000654089L 1.2 Use of the mixture: For in vitro diagnostic use. 1.3 Company identification: MANUFACTURER: DISTRIBUTOR EU: Instrumentation Laboratory Co. Via Roma, 103 180 Hartwell Road, 20040 Cavenago Brianza (Italy) Bedford, MA 01730-2443 (USA) Tel. +1 800 678 0710 DISTRIBUTOR US/CANADA: Fax +1 781 863 9928 DiaPharma Group, Inc. 8948 Beckett Rd. West Chester, OH 45069 (US) E-mail address of the competent person: infosds@mail.ilww.it 1.4 Emergency phone: +44 (0)3700 492 795 +1 215 207 0061 (USA and Canada) 22.. H HAAZZAARRD DSS IID DEEN NTTIIFFIICCAATTIIO ON N 2.1 Mixture classification (see also ch. 15) Classified: not dangerous according to 67/548/EEC and 1999/45/EEC Directives Classified: not hazardous according to 1272/2008/EC Regulation 2.2 Potential health and environmental effects Ingestion: May be harmful if swallowed. Inhalation exposure: May cause irritation. Contact with skin: May cause irritation. Contact with eyes: May cause irritation. Sensitization: Might cause sensitization by inhalation or skin contact. Environmental exposure: Might cause adverse effects for the environment. 33.. CCO OM MPPO OSSIITTIIO ON N//IIN NFFO ORRM MAATTIIO ON N O ON N IIN NGGRREED DIIEEN NTTSS Composition: solid containing organic components. 3.1 Hazardous components: Name EINECS/ CAS n° Conc. %

Precautionary statements

none Other labeling details:  8.6% percent of this mixture consist of ingredient(s) of unknown toxicity for health and aquatic environment. Wear suitable protective clothing, gloves and eye/face protection. Authorization: no Restriction: no 1166.. O OTTH HEERR IIN NFFO ORRM MAATTIIO ON N

R-phrase(s)

R36/38: Irritating to eyes and skin. R36/37/38: Irritating to eyes, respiratory system and skin.