Pseudotyped Luciferase rSARS-CoV-2 Spike

SECTION 2: Hazards identification

This product should be handled as a biohazardous material under Biosafety Level 2 or Enhanced Biosafety Level 2 Containment. Contact your local institutional biosafety office for accurate regional information. Pathogenicity: Lentivirus-derived non-replicating vectors derived from HIV 1 do not encode the viral proteins or genome required for viral replication. Replication-defective lentiviral vectors are not known to cause any diseases in humans or animals. However, they can integrate into the cell genome and thus pose some risk of insertional mutagenesis.SARS-CoV 2 reporter pseudotyped virus have a limited cell tropism based on the Spike protein that mediates fusion, and infect only permissive cells (e.g. that express the receptor ACE-2). The Pseudotyped Luciferase rSARS-CoV-2 Spike is derived from a second-generation 3 plasmid system that requires 3 recombination events to generate replication competent lentivirus. Gag and pol genes are together on the same plasmid as tat and rev. The HIV1 accessory genes vif, vpr, vpu, nef are absent. The HIV-1 envelope gene is also absent. The genome packaged into the virus does not encode viral proteins. Bio-Hazard: As per guidelines recommended by the NIH, replication-incompetent lentiviral particles are to be handled as Risk Group-Level 2 (RGL2) (https://osp.od.nih.gov/wpcontent/uploads/Lenti_Containment_Guida nce.pdf) Composition Hazard: Buffers: NR: BDH Hazard Class System. Corresponds to UN Hazard Classification. Chelatings: See individual product datasheet. Preservatives: See individual product datasheet. Protective agents: See individual product datasheet. Stability And Reactivity: Thermal decomposition: > 275°C (527 deg F). Conditions to avoid: Humidity; Contact with water, acids, heavy metals (lead, copper), metal salt, bromine, methylene chloride, carbon disulfide, sulfuric acid, halogenated, hydrocarbons. Reaction Hazard: May liberate toxic gas. Decomposition Hazard: Nitrous gas Caution: MAY BE HARMFUL IF SWALLOWED. Do not ingest. Wash thoroughly after handling. Do not get in eyes, on skin, or on clothing.

Principle Routes of Exposure

Dermal contact. Eye contact. Inhalation. Ingestion. Eyes: Eye contact may cause transient eye irritation. (BSA) Skin: Causes skin irritation. May be fatal if absorbed through the skin. Inhalation: Highly toxic if swallowed (R28): Causes irritation of mouth, pharynx, gullet and gastrointestinal tract. Mucosal irritation, cough, dyspnea, pulmonary edema after lag time. Ingestion: Ingestion may cause allergic reaction characterized by a rash. (BSA) Sensitizing effects: Not known. Industrial handling: Handle as if capable of transmitting infectious agents. Replication- defective lentiviral vectors are not known to cause disease in humans or animals. However, lentiviruses can integrate into the host cell genome and may pose some risk of insertional mutagenesis)