p53 Transcription Factor Activity Assay Kit

Hazard statements (CLP)

H227, H302, H303, H314, H315, H319, H412

Code

Statements

H227

Combustible liquid

SECTION 2: Hazards identification

NFPA Rating Component Description Volume Safety Information Plate Coated with DNA Probes -- 1 No hazards Binding Buffer (5X) Proprietary Buffer (contains Glycerol) 2.2 ml See below DTT (100 mM)

Contains

DTT 100 µl See below Protease Inhibitor Cocktail Liquid (contains DMSO) 20 μl See below p53 Primary Antibody Liquid 500 μl No hazards Antibody Diluent Buffer Liquid (contains BSA) 20 ml See below HRP Conjugate Stock Liquid 8 μl No hazards Wash Buffer (10X) Liquid (contains BSA) 27 ml See below Competitor Oligo (20 pmole) Liquid 25 μl No hazards Non-Competitor Oligo (20 pmole) Liquid 25 μl No hazards TMB Substrate Liquid 10 ml No hazards Stop Solution Liquid (contains Sulfuric acid) 6 ml See below Positive Control Liquid 50 μl No hazards Plate Sealing Film -- 2 No hazards DTT: Emergency Overview

2.3 Other hazards

Target Organ Effect, Toxic by ingestion, Irritant Target Organs: Central nervous system Not available Target Organs: Eyes, Skin Combustible liquid, Target organ effect Target Organs: Eyes, Skin

2.1 Classification of the substance or mixture

Acute toxicity, Oral (Category 5) Skin irritation (Category 2) Eye irritation (Category 2A) Acute Toxicity; Oral, Category 4 Not available Acute toxicity, Oral (Category 5) Skin corrosion (Category 1A) Serious eye damage (Category 1) Acute aquatic toxicity (Category 3) Chronic aquatic toxicity (Category 3) Flammable liquids (Category 4)

Signal word

Warning Warning Warning Danger Warning

Hazard statements

H303 May be harmful if swallowed H315 Causes skin irritation H319 Causes serious eye irritation H302: Harmful if swallowed. Not available H303 May be harmful if swallowed. H314 Causes severe skin burns and eye damage. H412 Harmful to aquatic life with long lasting effects. H227 Combustible liquid

Precautionary statements

P305+P351+P338 IF IN EYES Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P264 Wash {hands} thoroughly after handling P301+312 IF SWALLOWED Call a POISON CENTER or doctor/physician if you feel unwell P330: Rinse mouth. none P273 Avoid release to the environment P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P310 Immediately call a POISON CENTER or doctor/ physician. none

Health hazards

Flammability: 0 0 Flammability: 1 Flammability: 0 Flammability: 2

Physical hazards

0 0 2 0

NFPA Ratings

Fire: 0 Reactivity Hazard: 0 Fire: 0 Reactivity Hazard: 0 Fire: 0 Reactivity Hazard: 2 Special hazard: W Fire: 2 Reactivity Hazard: 0

Potential health effects

Inhalation: May be harmful if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: Toxic if swallowed. Bovine Serum Albumin (BSA): Emergency Overview: Harmful if swallowed. Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation or skin absorption. May cause eye, skin, or respiratory system irritation. To the best of our knowledge, the toxicological properties have not been thoroughly investigated Inhalation: May be harmful by inhalation. Material may be irritating to the mucous membranes and upper respiratory tract. Skin: Harmful if absorbed through skin. May cause skin irritation. Eyes: Cause eye irritation. Ingestion: Harmful if swallowed Glycerol: Emergency Overview Inhalation: Low hazard for usual industrial handling. Inhalation of a mist of this material may cause respiratory tract irritation. Skin: May cause skin irritation. Low hazard for usual industrial handling Eyes: May cause eye irritation. Ingestion: Ingestion of large amounts may cause gastrointestinal irritation. Low hazard for usual industrial handling. May cause headache. Sulfuric acid (H2SO4): Emergency Overview Inhalation: May be harmful if inhaled. Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin burns. Causes skin irritation. Eyes: Causes eye burns. Causes severe eye DMSO: Emergency Overview Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed.

HMIS® ratings

Fire: 0 Reactivity Hazard: 0

Hazard pictograms

none

Medical Conditions Generally Aggravated by Exposure

Avoid contact w/DMSO solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body.