Mitochondrial Complex I Activity Assay

Hazard statements (CLP)

H227, H301, H312, H315, H319, H335, H400

Code

Statements

H227

Combustible liquid

SECTION 2: Hazards identification

NFPA Rating Component Description Volume Safety Information Complex I Assay Buffer Liquid 25 ml No hazards NADH -- 1 vial No hazards Decylubiquinone

Contains

Decylubiquinone 1 vial See below Complex I Dye -- 1 vial No hazards Complex I Inhibitor Rotenone and DMSO 100 µl See below Rotenone Clear 96-well half area -- 1 plate No hazards plate Rotenone: Emergency Overview

2.3 Other hazards

Target organ effect, Toxic by ingestion, Harmful by skin absorption, Irritant Irritant Combustible liquid, Target organ effect Target Organs: Eyes, Skin

2.1 Classification of the substance or mixture

Acute toxicity, Oral (Category 3) Acute toxicity, Dermal (Category 4) Skin irritation (Category 2) Eye irritation (Category 2A) Specific target organ toxicity – single exposure (Category 3) Acute aquatic toxicity (Category 1) Skin irritation (Category 2) Eye irritation (Category 2A) Specific target organ toxicity – single exposure (Category 3) Flammable liquids (Category 4)

Signal word

Danger Warning Warning

Hazard statements

H301 Toxic if swallowed. H312 Harmful in contact with skin. H315 Causes skin irritation. H319 Causes serious eye irritation. H335 May cause respiratory irritation. H400 Very toxic to aquatic life. H315 Causes skin irritation. H319 Causes serious eye irritation. H335 May cause respiratory irritation. H227 Combustible liquid

Precautionary statements

P261 Avoid breathing dust/fume/gas/mist/vapors/spray P273 Avoid release to the environment. P280 Wear protective gloves/protective clothing. P301+P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P261 Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P264 Wash skin thoroughly after handling. P271 Use only outdoors or in a well-ventilated area. P280 Wear protective gloves/ eye protection/ face protection. P302 + P352 IF ON SKIN: Wash with plenty of soap and water. P304+P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P312 Call a POISON CENTER or doctor/ physician if you feel unwell. P332 + P313 If skin irritation occurs: Get medical advice/ attention. P337 + P313 If eye irritation persists: Get medical advice/ attention. P362 Take off contaminated clothing and wash before reuse. P501 Dispose of contents/ container to an approved waste disposal plant. none

Health hazards

2 Flammability: 0 2 Fire: 0 Reactivity Hazard: 0 2 Flammability: 0 2 Fire: 0 Reactivity Hazard: 0 0 Flammability: 2 0 Fire: 2 Reactivity Hazard: 0

Physical hazards

0 0 0

Potential health effects

Inhalation: May be harmful if inhaled. Causes respiratory tract irritation. Skin: Harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: Toxic if swallowed. Decylubiquinone: Emergency Overview Inhalation: May be harmful if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: May be harmful if swallowed. DMSO: Emergency Overview Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed.

Hazard pictograms

none

Medical Conditions Generally Aggravated by Exposure

Avoid contact w/DMSO solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body.