HDAC1 Immunoprecipitation (IP) & Activity Assay Kit

Hazard statements (CLP)

H226, H227, H302, H315, H315+H320, H317, H319, H334, H335, H400, H410

Code

Statements

H226

Flammable liquid and vapour

SECTION 2: Hazards identification

OSHA Hazards Component Description Volume Safety Information HDAC Assay Buffer Proprietary Buffer 9 ml No hazards Cell Lysis Buffer Proprietary Buffer (contains Tergitol) 100 ml See below HDAC Substrate In DMSO 100 µl See below Developer Liquid (contains Trypsin) 500 µl See below AFC (7-amino-4-trifluoromethyl In DMSO 100 µl See below coumarin) Standard (1 mM) Rabbit HDAC1 Antibody Affinity purified polyclonal antibody (contains Glycerol & 150 µl See below BSA) Rabbit IgG (Control Antibody) Control Antibody (contains Glycerol & BSA) 75 µl See below Protein A/G Sepharose Beads (50%

Contains

Ethanol 650 µl See below slurry in 20% ethanol/H2O) Positive Control (Jurkat Cell Lysate, Lyophilized 1 vial No hazards lyophilized) Tergitol: Emergency Overview

2.1 Classification of the substance or mixture

Skin irritation (Category 2), H315 Skin sensitization (Sub-category 1A), H317 Short-term (acute) aquatic hazard (Category 1), H400 Long-term (chronic) aquatic hazard (Category 1), H410 Flammable liquids (Category 4) Skin irritation (Category 2) Eye irritation (Category 2A) Respiratory sensitization (Category 1) Skin sensitization (Category 1) Specific target organ toxicity – single exposure (Category 3) Flammable liquids (Category 3) Skin irritation (Category 2) Eye irritation (Category 2B) Specific target organ toxicity – single exposure (Category 3) Acute Toxicity; Oral, Category 4 Not available

Signal word

Warning Warning Danger Warning Warning Warning

Hazard statements

H315 Causes skin irritation. H317 May cause an allergic skin reaction. H410 Very toxic to aquatic life with long lasting effects. H227 Combustible liquid H315 Causes skin irritation. H317 May cause an allergic skin reaction. H319 Causes serious eye irritation. H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled. H335 May cause respiratory irritation. H226 Flammable liquid and vapor. H315+H320 Causes skin and eye irritation. H335 May cause respiratory irritation. H302: Harmful if swallowed. Not available

Precautionary statements

P261 Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P264 Wash skin thoroughly after handling. P272 Contaminated work clothing must not be allowed out of the workplace. P273 Avoid release to the environment. P280 Wear protective gloves. P302 + P352 IF ON SKIN: Wash with plenty of soap and water. P333 + P313 If skin irritation or rash occurs: Get medical advice/ attention. P362 Take off contaminated clothing and wash before reuse. P391 Collect spillage. P501 Dispose of contents/ container to an approved waste disposalplant none P261 Avoid breathing dust/fumes/gas/mist/vapors/spray P280 Wear protective gloves/protective clothing. P302+P352 IF ON SKIN: Wash with plenty of soap and water. P304+P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P342+P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. P210 Keep away from heat/sparks/open flames/hot surfaces. No smoking P261 Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P264 Wash {hands} thoroughly after handling P301+312 IF SWALLOWED Call a POISON CENTER or doctor/physician if you feel unwell P330: Rinse mouth. none

Health hazards

0 Flammability: 0 0 Fire: 0 Reactivity hazard: 0 Inhalation: May be harmful if inhaled. Material is irritating to the tissue of the mucous membranes and upper respiratory tract.Harmful; if inhaled. May cause allergy or asthma symptoms or breathing difficulties. Skin: May be harmful if absorbed through skin. May cause an allergic skin reaction. Eyes: Causes eye irritation. Ingestion: Harmful if swallowed. To the best of our knowledge, the toxicological properties have not been thoroughly investigated DMSO: Emergency Overview 0 Flammability: 2 0 Fire: 2 Reactivity Hazard: 0 Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed. 2 Flammability: 0 2 Fire: 0 Reactivity Hazard: 0 Inhalation: May ber harmful if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: May be harmful if swallowed. Ethanol: Emergency Overview 2 Flammability: 2 2 Fire: 2 Reactivity Hazard: 0 Inhalation: May be harmful if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: May be harmful if swallowed. Bovine Serum Albumin (BSA): Emergency Overview: 1 Flammability: 0 1 Fire: 0 Reactivity Hazard: 0 Ingestion: Harmful if swallowed Glycerol: Emergency Overview 1 0 Flammability: 1 1 Fire: 0 Reactivity Hazard: 0 Inhalation: Low hazard for usual industrial handling. Inhalation of a mist of this material may cause respiratory tract irritation. Skin: May cause skin irritation. Low hazard for usual industrial handling Eyes: May cause eye irritation. Ingestion: Ingestion of large amounts may cause gastrointestinal irritation. Low hazard for usual industrial handling. May cause headache.

Physical hazards

0 0 0 0 0 0

2.3 Other hazards

Combustible liquid, Target organ effect Target Organs: Eyes, Skin Target organ effect, Skin and respiratory sensitizer, Irritant Target Organs: Lungs Combustible liquid, Target organ effect, Irritant, Carcinogen Target Organs: Nerves, Liver, Heart Not available Target Organs: Eyes, Skin

Hazard pictograms

none

Medical Conditions Generally Aggravated by Exposure

Avoid contact w/DMSO solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body. Trypsin: Emergency Overview

Potential health effects

Harmful if swallowed. Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation or skin absorption. May cause eye, skin, or respiratory system irritation. To the best of our knowledge, the toxicological properties have not been thoroughly investigated