Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric)

Hazard statements (CLP)

H227, H301, H301+H331, H303, H310, H315, H317, H318, H319, H331, H335, H360, H373, H400, H410

Code

Statements

H227

Combustible liquid

SECTION 2: Hazards identification

OSHA Hazards Component Description Volume Safety Information DHFR Assay Buffer Liquid (contains Tergitol) 35 ml See below DHFR Substrate Proprietary (contains Dihydrofolic acid & 2- 450 µl See below Mercaptoethanol) Dihydrofolate Reductase

Contains

Glycerol and DTT 1 vial See below NADPH Liquid 1 vial No hazards Methotrexate (10 mM) Methotrexate & DMSO 25 µl See below Tergitol: Emergency Overview

2.1 Classification of the substance or mixture

Skin irritation (Category 2), H315 Skin sensitization (Sub-category 1A), H317 Short-term (acute) aquatic hazard (Category 1), H400 Long-term (chronic) aquatic hazard (Category 1), H410 Skin irritation (Category 2), H315 Eye irritation (Category 2A), H319 Specific target organ toxicity - single exposure (Category 3), Respiratory system, H335 Flammable liquids (Category 4), H227 Acute toxicity, Oral (Category 3), H301 Acute toxicity, Inhalation (Category 3), H331 Acute toxicity, Dermal (Category 2), H310 Skin irritation (Category 2), H315 Serious eye damage (Category 1), H318 Skin sensitisation (Category 1), H317 Specific target organ toxicity - repeated exposure, Oral (Category 2), Liver, Heart, H373 Acute aquatic toxicity (Category 1), H400 Chronic aquatic toxicity (Category 1), H410 Acute toxicity, Oral (Category 5) Skin irritation (Category 2) Eye irritation (Category 2A) Skin irritation (Category 3) Eye irritation (Category 2B) Acute Toxicity Oral, Category 3 Skin Corrosion/Irritation, Category 2 Serious Eye Damage/Eye Irritation, Category 2 Toxic To Reproduction, Category 1B Flammable liquids (Category 4)

Signal word

Warning Warning Danger Warning none Danger Warning

Hazard statements

H315 Causes skin irritation. H317 May cause an allergic skin reaction. H410 Very toxic to aquatic life with long lasting effects. H315 Causes skin irritation. H319 Causes serious eye irritation. H335 May cause respiratory irritation. H227 Combustible liquid. H301 + H331 Toxic if swallowed or if inhaled. H310 Fatal in contact with skin. H315 Causes skin irritation. H317 May cause an allergic skin reaction. H318 Causes serious eye damage. H373 May cause damage to organs (Liver, Heart) through prolonged or repeated exposure if swallowed. H410 Very toxic to aquatic life with long lasting effects. H303 May be harmful if swallowed H315 Causes skin irritation H319 Causes serious eye irritation none H301: Toxic if swallowed. H315: Causes skin irritation. H319: Causes serious eye irritation. H360: May damage fertility or the unborn child H227 Combustible liquid

Precautionary statements

P261 Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P264 Wash skin thoroughly after handling. P272 Contaminated work clothing must not be allowed out of the workplace. P273 Avoid release to the environment. P280 Wear protective gloves. P302 + P352 IF ON SKIN: Wash with plenty of soap and water. P333 + P313 If skin irritation or rash occurs: Get medical advice/ attention. P362 Take off contaminated clothing and wash before reuse. P391 Collect spillage. P501 Dispose of contents/ container to an approved waste disposalplant P261 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray P264 Wash skin thoroughly after handling. P271 Use only outdoors or in a well-ventilated area. P280 Wear protective gloves/ eye protection/ face protection. P302 + P352 IF ON SKIN: Wash with plenty of soap and water. P304 + P340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P312 Call a POISON CENTER/doctor if you feel unwell. P321 Specific treatment (see supplemental first aid instructions on this label). P332 + P313 If skin irritation occurs: Get medical advice/ attention. P337 + P313 If eye irritation persists: Get medical advice/ attention. P362 Take off contaminated clothing and wash before reuse. P403 + P233 Store in a well-ventilated place. Keep container tightly closed. P405 Store locked up. P501 Dispose of contents/ container to an approved waste disposal plant. P210 Keep away from heat/sparks/open flames/hot surfaces. No smoking P260 Do not breathe dust/ fume/ gas/ mist/ vapours/ spray. P262 Do not get in eyes, on skin, or on clothing. P264 Wash skin thoroughly after handling. P270 Do not eat, drink or smoke when using this product. P271 Use only outdoors or in a well-ventilated area. P272 Contaminated work clothing should not be allowed out of the workplace. P273 Avoid release to the environment. P280 Wear protective gloves/ eye protection/ face protection. P301 + P310 + P330 IF SWALLOWED: Immediately call a POISON CENTER/doctor. Rinse mouth. P302 + P350 + P310 IF ON SKIN: Gently wash with plenty of soap and water. Immediately call a POISON CENTER or doctor/ physician. P302 + P352 IF ON SKIN: Wash with plenty of soap and water. P304 + P340 + P311 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. Call a POISON CENTER or doctor/ physician. P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Removecontact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor. P314 Get medical advice/ attention if you feel unwell. P333 + P313 If skin irritation or rash occurs: Get medical advice/ attention. P361 Remove/Take off immediately all contaminated clothing. P370 + P378 In case of fire: Use dry sand, dry chemical or alcohol-resistant foam for extinction. P391 Collect spillage. P403 + P233 Store in a well-ventilated place. Keep container tightly closed. P403 + P235 Store in a well-ventilated place. Keep cool. P405 Store locked up. P501 Dispose of contents/ container to an approved waste disposal plant. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P305+P351+P338 IF IN EYES Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P201 Obtain special instructions before use P202: Do not handle until all safety precautions have been read and understood. P264: Wash {hands} thoroughly after handling. P280: Wear {protective gloves/protective clothing/eye protection/face protection}. P301+310 IF SWALLOWED Immediately call a POISON CENTER or doctor/physician P302+352 IF ON SKIN Wash with plenty of soap and water P305+351+338 IF IN EYES Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308+313 IF exposed or concerned Get medical attention/advice P330: Rinse mouth. P332+313: If skin irritation occurs, get medical advice/attention. P337+313: If eye irritation persists, get medical advice/attention. P362+364: Take off contaminated clothing and wash it before reuse. none

Health hazards

0 Flammability: 0 0 Fire: 0 Reactivity hazard: 0 Flammability: Fire: Reactivity hazard: Flammability: Fire: Reactivity hazard: 2 Flammability: 0 2 Fire: 0 Reactivity Hazard: 0 1 Flammability: 1 1 Fire: 1 Reactivity Hazard: 0 0 Flammability: 2 0 Fire: 2 Reactivity Hazard: 0 0 Flammability: 2 0 Fire: 2 Reactivity Hazard: 0

Physical hazards

0 0 0 0 0

Potential health effects

Inhalation: May be harmful if inhaled. Material is irritating to the tissue of the mucous membranes and upper respiratory tract.Harmful; if inhaled. May cause allergy or asthma symptoms or breathing difficulties. Skin: May be harmful if absorbed through skin. May cause an allergic skin reaction. Dihydrofolic acid: Emergency Overview Inhalation: May be harmful if inhaled. Material is irritating to the tissue of the mucous membranes and upper respiratory tract.Harmful; if inhaled. May cause allergy or asthma symptoms or breathing difficulties. Skin: May be harmful if absorbed through skin. May cause an allergic skin reaction. 2-Mercaptoethanol: Emergency Overview Inhalation: May be harmful if inhaled. Material is irritating to the tissue of the mucous membranes and upper respiratory tract.Harmful; if inhaled. May cause allergy or asthma symptoms or breathing difficulties. Skin: May be harmful if absorbed through skin. May cause an allergic skin reaction. DTT: Emergency Overview Inhalation: May be harmful if inhaled. Causes respiratory tract irritation. Skin: May be harmful if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Ingestion: Toxic if swallowed. Eyes: Causes eye irritation. Ingestion: Toxic if swallowed. Glycerol: Emergency Overview Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed. Methotrexate: Emergency Overview Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed. Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed.

2.3 Other hazards

Target Organ Effect, Toxic by ingestion, Irritant Target Organs: Central nervous system Target Organ Effect Target Organs: Kidney Combustible liquid, Target organ effect Target Organs: Eyes, Skin

Hazard pictograms

none

Medical Conditions Generally Aggravated by Exposure

Avoid contact w/DMSO solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body. DMSO: Emergency Overview Avoid contact w/DMSO solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body.