ELISpot Blue Color Module (Strep-AP and BCIP-NBT), 5 Plate

Hazard statements (CLP)

H225, H301, H311, H317, H319, H331, H371

Code

Statements

H225

Highly Flammable liquid and vapour

SECTION 2: Hazards identification

Hazard categories

6.1) Packing group II IATA: UN Number UN 1992 Proper Shipping Name Flammable liquid, toxic, n.o.s. (Methanol, Acetone, Isopropyl Alcohol) (6.1) Packing group II IMDG: UN Number UN 1992 Proper Shipping Name Flammable liquid, toxic, n.o.s. (Methanol, Acetone, Isopropyl Alcohol) (6.1) Packing group II Marine Pollutant No Regulations US Federal and State Regulations TSCA (Toxic Substances Control Act): Methanol is listed. Acetone is listed. Isopropyl alcohol is listed SARA 313: Methanol is listed. Acetone is listed. Isopropyl alcohol is not listed SARA 311/312 Hazards: Methanol: Acute Health Hazard. Acetone: Acute Health Hazard. Isopropyl alcohol: Acute Health Hazard None ▪ Land transport ADR/RID (cross-border): Not regulated. ▪ Maritime transport IMDG: Not regulated. ▪ Marine pollutant: No ▪ Air transport ICAO-TI and IATA-DGR: Not regulated. ▪ Transport/Additional information: Not dangerous according to the above specifications. 15. Regulations: ▪ US Federal and State Regulations TSCA (Toxic Substances Control Act): On TSCA Inventory SARA 313 Components: Not listed SARA 311/312 Hazards: Acute Health Hazard

Precautionary statements

P261: Avoid breathing mist. P272: Contaminated work clothing should not be allowed out of the workplace. P280: Wear protective gloves.

Response

IF ON SKIN: Wash with plenty of soap and water. If skin irritation or rash occurs: get medical attention. Wash contaminated clothing before reuse.

EU Classification

Directive 67/548/EEC The product is classified as a skin sensitizer according to Directive 1999/45/EC and its amendments. Hazard Symbol / R-Phrase / S-Phrase R43 May cause sensitization by skin contact

2.3 Other hazards

none 3. Information on Ingredients: Description: Proclin 300

Contains

CAS No. EC-No. Index-No. Modified Alkyl Carboxylate - <0.01% Mixture of 5-Cloro-2- 55965-84-9 - 613-167-00-5 <0.01% Methyl-4-Isothiazolin-3- One (26172-55-4) and - Methyl-4-Isothiazolin-3- One (2682-20-4) Modified Glycol - ≤1.6% 4. First Aid Measures: ▪ IF IN EYES: Rinse thoroughly with water for at least 15 minutes and immediately consult a physician. ▪ IF ON SKIN (or hair): Immediately take off contaminated clothing or shoes. Wash with plenty of soap and water. Consult a physician. ▪ IF INHALED: If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician. ▪ IF SWALLOWED: Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Immediately consult a physician. 5. Fire Fighting Measures: ▪ Suitable extinguishing agents: Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. ▪ Special precautions for fire-fighters: Self contained breathing apparatus and full protective clothing must we worn in case of fire. 6. Accidental Release Measures: ▪ Person-related safety precautions: Use appropriate personal protective equipment to prevent contamination of skin, eyes and personal clothing. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. ▪ Measures for environmental protection: Keep away from drains. ▪ Measures for containment and cleaning: Soak up with inert absorbent material and dispose of as hazardous waste. Keep in suitable, closed containers for disposal. 7. Handling and Storage: ▪ Precautions for safe handling: Avoid contact with skin and eyes. Avoid inhalation of vapour or mist. Use normal measures for preventive fire protection. ▪ Conditions for safe storage: Store in a cool, dry place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. 8. Exposure Controls and Personal Protection: ▪ Control parameters: Contains no substances with occupational exposure limit values. ▪ Individual protection measures: Wash hands thoroughly after handling chemical products and before eating, smoking or using the toilet. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Eye/face protection: Wear approved safety goggles. Skin/hand protection: Handle with protective gloves, plastic or rubber. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. Body protection: Wear suitable protective clothing as protection against splashing or contamination. Other skin protection: Appropriate footwear and any additional skin protection measures should be selected based on the task being performed and the risks involved. Respiratory protection: In case of inadequate ventilation, use a suitable respirator. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). 9. Physical and Chemical Properties: ▪ Appearance: Clear Liquid Upper/lower flammability or explosive limits: Not available ▪ Odor: Not available Vapor density Not available ▪ Odor threshold: Not available Vapor pressure: Not available ▪ pH: Not available Relative density: 1.03 g/cm3 ▪ Melting point/freezing point: -40º C Solubility in/Miscibility with Water: Soluble ▪ Boiling point/Boiling range: 189º C Partition coefficient noctanol/water Not available ▪ Flash point: 118º C – closed cup Auto igniting: Not available ▪ Evaporation rate: Not available Decomposition temperature: Not available ▪ Flammability (solid, gas): Not available Viscosity: Not available 10. Stability and Reactivity: ▪ Chemical Stability: Stable under recommended storage conditions. ▪ Conditions to avoid: Strong oxidizing agents, reducing agents, Amines, Mercaptans ▪ Hazardous decomposition products: Hazardous decomposition products formed under fire conditions – Carbon oxides, nitrogen oxides (NOx), Sulphur oxides, Hydrogen chloride gas.

TOXICOLOGICAL INFORMATION

▪ Acute toxicity: LD50 Oral – rat – 862 mg/kg LD50 Dermal – rabbit- 2800 mg/kg ▪ Skin corrosion/irritation: Can cause severe burns. Skin – rabbit – Corrosive ▪ Serious eye damage/irritation: Rabbit – Corrosive to eyes ▪ Respiratory or skin sensitization: May cause allergic skin reaction. ▪ Germ cell mutagenicity: No data available ▪ Carcinogenicity: No data available ▪ Reproductive toxicity: No data available ▪ Specific target organ toxicity (STOT) -single exposure: No data available ▪ Specific target organ toxicity (STOT) -repeated exposure: No data available

Aspiration hazard

Can cause severe burns ▪ Information on likely routes of exposure: Routes of entry anticipated; oral, dermal, inhalation. ▪ Symptoms related to the physical, chemical and toxicological characteristics: Inhalation: Harmful if inhaled. Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. Ingestion: Harmful if swallowed. Causes burns. Skin contact: Harmful if absorbed through skin. Causes burns. Eye contact: Causes eye burns. ▪ Delayed and immediate effects and also chronic effects from short and long term exposure: Short term exposure Potential immediate effects Not available. Potential delayed effects Not available Long term exposure Potential immediate effects Not available. Potential delayed effects Allergic contact dermatitis ▪ Effects of chronic exposure: Proclin 300 at levels greater than or equal to 0.1% is not identified as probable, possible or a confirmed human carcinogen by IARC. ▪ Numerical measures of toxicity: Not available

Further information

NA ▪ Hazard Symbol / R-Phrase / S-Phrase: R43 May cause sensitization by skin contact. ▪ Notice to reader: To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

ECOLOGICAL INFORMATION

▪ Ecotoxicity: No data available ▪ Biodegradability: No data available. ▪ Bioaccumulative potential: No data available. ▪ Mobility in soil: No data available.

Other hazards

Toxic to aquatic organisms

Disposal

Considerations ▪ Disposal methods: Dispose of waste in accordance to applicable national, regional, or local regulations. ▪ Contaminated packaging: Dispose in the same manner as unused product. ▪ Special precautions: Dispose of small amounts of spilled material as described in section 6. Large spills must be dealt with separately by qualified disposal personnel. Avoid dispersal of spilled material to soil, waterways, drains and sewers. 14. Transport Information

UN Number

None DOT regulations

Principle Routes of Exposure

Inhalation; ingestion or skin IF EXPOSED OR CONCERNED: Get medical advice/attention.

2.1 Classification of the substance or mixture

Acute Toxicity, Oral 3 Acute Toxicity, Inhalation 3 Acute Toxicity, Dermal 3 STOT, Single Exposure, 2 Flammable Liquid, 2 Eye Irritation, 2A Signal Word: DANGER ▪ Hazard Statements: H301: Toxic if swallowed. H311: Toxic in contact with skin. H331: Toxic if inhaled. H371: May cause damage to organs. H225: Highly flammable liquid and vapour. H319: Causes serious eye irritation. ▪ Precautionary Statements: P264: Wash hands thoroughly after handling. P270: Do not eat, drink or smoke when using this product. P280: Wear protective gloves/protective clothing/eye protection/face protection. P260: Do not breath dust/ fume/gas/mist/ vapours/spray. P271: Use only outdoors or in a well-ventilated area. P210: Keep away from heat/sparks/ open flames/hot surfaces. No smoking. P233: Keep container tightly closed. P241: Use explosion-proof electrical equipment. P242: Use only non-sparking tools. P243: Take precautionary measure against static discharge. Responses: IF SWALLOWED: Immediately call a Poison Control Center or physician. See specific treatment in this MSDS. Rinse mouth. IF ON SKIN: Wash with plenty of soap and water. Call a POISON CENTER or doctor/physician if you feel unwell. See specific treatment in this MSDS. Remove/take off immediately all contaminated clothing. Wash contaminated clothing before reuse. Rinse skin with water/shower. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. Call a POISON CENTER or doctor/physician. See specific treatment in this MSDS. IF EXPOSED OR CONCERNED: Call a POISON CENTER or doctor/physician. IN CASE OF FIRE: Use alcohol resistant foam, carbon dioxide, or dry chemical to extinguish. ▪ Classification according to EU Directives 67/548/EEC or 1999/45/EC: Xn: Harmful. Xi: Irritant. F: Highly Flammable. ▪ R-phrases: R20/21/22: Harmful by inhalation, in contact with skin and if swallowed. R68/20/21/22: Harmful: possible risk of irreversible effect through inhalation, in contact with skin and if swallowed. R11: Highly flammable. R66: Repeated exposure may cause skin dryness or cracking. R67: Vapours may cause drowsiness and dizziness. ▪ S-phrases: S36/37: Wear suitable protective clothing and gloves. S16: Keep away from sources of ignition-No smoking. S45: In case of accident or if you feel unwell, seek medical advice immediately (show label if possible). S51: Use only in well-ventilated areas. S1/2: Keep locked up and out of reach of children. S7: Keep container tightly closed. S9: Keep container in a well ventilated place. S16: Keep away from sources of ignition – No smoking. S24/25: Avoid contact with skin and eyes. S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. ▪ Special Hazards: none. 3. Information on Ingredients: ▪ Description: BCIP/NBT Chromogen Solution Contains CAS No. EC-No. Index-No. Content Methanol 67-56-1 200-369-6 603-001-00-X 3 - 10% Acetone 67-64-1 200-662-2 606-001-00-8 3 – 10% Isopropyl Alcohol 67-63-0 200-661-7 603-117-00-0 3 – 10% 4. First Aid Measures: ▪ After inhalation: Move person into fresh air. If not breathing, give artificial respiration. Consult a physician. ▪ After skin contact: Wash off with soap and plenty of water. Take victim immediately to a hospital. Consult a physician. ▪ After eye contact: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Show this MSDS. ▪ After swallowing: Never give anything by mouth to an unconscious person. Rinse mouth with water. Call a physician. Show this MSDS. ▪ Most important symptoms and effects, both acute and delayed: Unknown ▪ Indication of any immediate medical attention and special treatment needed: No data available 5. Fire Fighting Measures: ▪ Suitable extinguishing agents: Dry chemical, carbon dioxide, water spray or alcohol-resistant foam. Do not extinguish fire unless flow can be stopped. Use water in flooding quantities as fog. Solid streams of water may be ineffective. Cool all affected containers with flooding quantities of water. Apply water from as far a distance as possible. ▪ Special hazards arising from the substance or mixture: Emits toxic fumes under fire conditions. Carbon oxides. ▪ Protective equipment: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. ▪ Further information: Use water spray to cool unopened containers. 6. Accidental Release Measures: ▪ Personal precautions, protective equipment and emergency procedures: Wear respiratory protection. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Remove all sources of ignition. Evacuate all personnel to safe areas. Beware of vapors accumulating to form explosive concentrations. Vapors can accumulate in low areas. ▪ Environmental precautions: Prevent further leakage or spillage is safe to do so. Do not let product enter drains. Discharge into the environment must be avoided. ▪ Methods and materials for containment and cleaning up: Contain spillage, and then collect with an electrically protected vacuum cleaner or by wet-brushing and place in container for disposal according to local regulations. 7. Handling and Storage: ▪ Precautions for safe handling: Avoid contact with skin and eyes. Avoid inhalation of vapor or mist. Keep away from sources of ignition – No Smoking. Take measures to prevent the buildup of electrostatic charge. ▪ Conditions for safe storage, including incompatibilities: Store locked up. Store in a cool place. Keep container tightly closed in a dry and well ventilated place. 8. Exposure Controls and Personal Protection: ▪ Control parameters: Component: Methanol: UK EH40 WEL Workplace Exposure Limits: STEL 250 ppm, 333 mg/m3. TWA 200 ppm, 266 mg/m3. NIOSH REL: STEL 250 ppm, 325 mg/m3. TWA 200 ppm, 260 mg/m3. Acetone: UK EH40 WEL Workplace Exposure Limits: STEL 1,500 ppm, TWA 750 ppm. NIOSH REL: STEL 250 ppm, 590 mg/m3. TWA 21,000 ppm, 2,400 mg/m3. Isopropyl Alcohol UK EH40 WEL Workplace Exposure Limits: STEL 1225 ppm, TWA 980 ppm. NIOSH REL: STEL 500 ppm, 1,225 mg/m3. TWA 400 ppm, 980 mg/m3. ▪ Appropriate engineering controls: Wear protective gloves/protective clothing/eye protection/face protection. Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling product. Personal Protective Equipment: Eye and Face Protection: Tightly fitting safety goggles. Face shield (8-inch minimum). Skin Protection: Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove’s outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. Body Protection: Complete suit protection against chemicals, flame retardant antistatic protective clothing, the type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace. Respiratory Protection: Where risk assessment shows air-purifying respirators are appropriate use full-face respirator. 9. Physical and Chemical Properties: ▪ Appearance: Liquid Upper/lower flammability or explosive limits: Not available ▪ Odor: Pungent odor Vapor density : Not available ▪ Odor threshold: Not available Vapor pressure: Not available ▪ pH: Not available Relative density: Not available ▪ Melting point/freezing point: Not available. Boiling point/Boiling range: Not available ▪ Partition coefficient: noctanol/water: Not available Auto igniting: Not available ▪ Flash point: Not available Decomposition temperature: Not available ▪ Water solubility: Soluble Viscosity: Not available ▪ Evaporation rate: Not available ▪ Flammability (solid, gas): Not available 10. Stability and Reactivity: ▪ Reactivity: Strong oxidizers, alkalis and acids, phenols, urea, acetaldehyde, chlorine, ethylene oxide, isocyanates. ▪ Chemical Stability: Contains the following stabilizer: Methanol (>3 - <10%). ▪ Possibility of hazardous reactions: no data available. ▪ Conditions to avoid: Heat, flames, sources of ignition. ▪ Incompatible materials: Strong oxidizers, alkalis and acids, phenols, urea, acetaldehyde, chlorine, ethylene oxide, isocyanates. ▪ Hazardous decomposition products: no data available. 11. Toxicological Information: ▪ Acute toxicity: Methanol: rat oral – LD50 = 6200 mg/kg; mouse oral – LD50 = 400 mg/kg. Acetone: rat oral – LD50 = 9,800 mg/kg; mouse oral – LD50 = 3,000 mg/kg. Isopropyl Alcohol – rat oral – LD50 = 4,420 mg/kg; mouse oral – LD50 = 4,800 mg/kg. ▪ Skin corrosion/irritation: Methanol: rabbit dermal – LD50 = 16,000 mg/kg. Acetone: rabbit dermal - LD50 = 20 mg/kg Isopropyl Alcohol: rabbit dermal 500 mg/24 hr (mild). ▪ Serious eye damage/irritation: Methanol: no data available. Acetone: rabbit eye – irritation after 24 hr. Isopropyl Alcohol: rabbit eye 0.1 mL of 70% solution (severe eye irritation). ▪ Respiratory or skin sensitization: Methanol: no data available Acetone: Isopropyl Alcohol: Causes respiratory irritation at 400 ppm. High concentrations are toxic. ▪ Germ cell mutagenicity: no data available. ▪ Carcinogenicity: no data available. ▪ Reproductive toxicity: no data available. ▪ Specific target organ toxicity (STOT) -single exposure: Methanol: acute intoxication causes CNS depression as observed by narcosis, in coordination, lethargy, drowsiness and prostration. A latent period follows the initial intoxication in which formic acid accumulates in the body causing metabolic acidosis. Acetone: pulmonary congestion, seizures, edema. Isopropyl Alcohol: High concentrations cause nausea, headache, lightheadedness, drowsiness, ataxia and deep narcosis. ▪ Specific target organ toxicity (STOT) -repeated exposure: no data available. ▪ Aspiration hazard: No data available ▪ Information on likely routes of exposure: Routes of entry anticipated; oral, dermal, inhalation. ▪ Symptoms related to the physical, chemical and toxicological characteristics: Inhalation: Causes conjunctivitis, headache, giddiness, insomnia, gastric disturbances, bilateral blindness, nausea, headache, lightheadedness, drowsiness, ataxia and deep narcosis. Ingestion: Causes acute intoxication followed by a latent period of metabolic acidosis and severe abdominal, leg and back pain, followed by blindness. Skin contact: Irritation and redness. Eye contact: Causes serious eye irritation which can lead to blindness. ▪ Delayed and immediate effects and also chronic effects from short and long term exposure: Acute intoxication is manifested by signs of narcosis. This is followed by formic acid accumulation in the body causing metabolic acidosis. Severe abdominal pain and severe abdominal, leg and back pain, followed by blindness. 12. Ecological Information: ▪ Ecotoxicity: Methanol: low acute toxicity to aquatic organisms. LD50 > 100 mg/L. Acetone: Oncorhynchus mykiss (rainbow trout) LD50 = 5,540 mg/L/96 hr. Isopropyl Alcohol: no data available. ▪ Biodegradability: Reacts with nitrogen dioxide in polluted atmospheres to product methyl nitrite. It is a volatile organic substance. ▪ Bioaccumulative potential: no data available. ▪ Mobility in soil: no data available. ▪ Other adverse effects: As a volatile organic compound, it can contribute to the formation of photochemical smog in the presences of other volatile organic compounds. 13. Disposal Considerations: ▪ Disposal methods: Dispose of waste in accordance to applicable national, regional, or local regulations. Contact a licensed professional waste disposal service to dispose of this material. ▪ Contaminated packaging: Dispose in the same manner as unused product. 14. Transport Information: ▪ ADR/RID ADN/ADNR IMDG IATA/DOT ADR/DOT/: UN Number: UN 1992 RID Proper Shipping Name: Flammable liquid, toxic, n.o.s. (Methanol, Acetone, Isopropyl Alcohol) Hazard class: 3 (6.1) Packing group: II IATA: UN Number: UN 1992 Proper Shipping Name: Flammable liquid, toxic, n.o.s. (Methanol, Acetone, Isopropyl Alcohol) Hazard class: 3 (6.1) Packing group: II IMDG: UN Number: UN 1992 Proper Shipping Name: Flammable liquid, toxic, n.o.s. (Methanol, Acetone, Isopropyl Alcohol) Hazard class: 3 (6.1) Packing group: II Marine Pollutant: No 15. Regulations: ▪ US Federal and State Regulations TSCA (Toxic Substances Control Act): Methanol is listed. Acetone is listed. Isopropyl alcohol is listed. SARA 313: Methanol is listed. Acetone is listed. Isopropyl alcohol is not listed. SARA 311/312 Hazards: Methanol: Acute Health Hazard. Acetone: Acute Health Hazard. Isopropyl alcohol: Acute Health Hazard. CERCLA Reportable Quantity: Methanol: 5,000 pounds. Acetone: 5,000 pounds. Isopropyl alcohol is not listed. California Proposition 65: WARNING: This product can expose you to chemicals including ethylene glycol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov. 16. Other Information: ▪ R-phrases: R20/21/22: Harmful by inhalation, in contact with skin and if swallowed. R68/20/21/22: Harmful: possible risk of irreversible effect through inhalation, in contact with skin and if swallowed. R11: Highly flammable. R66: Repeated exposure may cause skin dryness or cracking. R67: Vapours may cause drowsiness and dizziness. ▪ S-phrases: S36/37: Wear suitable protective clothing and gloves. S16: Keep away from sources of ignition-No smoking. S45: In case of accident or if you feel unwell, seek medical advice immediately (show label if possible). S51: Use only in well-ventilated areas. S1/2: Keep locked up and out of reach of children. S7: Keep container tightly closed. S9: Keep container in a well ventilated place. S16: Keep away from sources of ignition – No smoking. S24/25: Avoid contact with skin and eyes. S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. ▪ Notice to reader: To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. 2. Hazard Identification: ▪ Classification: Regulation (EC) No. 1272/2008 (CLP/EU-GHS), 29 CFR 1910.1200 (OSHA): Some components indicated above contain Proclin 300: Skin Sens. 1; H317 – May cause an allergic skin reaction. ▪ Signal Word: WARNING