Cell-Based ELISA Kits

SECTION 2: Hazards identification

Potential hazards Some kit reagents are not considered to be hazardous according to 29CFR 1910.1200(d). They are mixtures that contain less than 1% hazardous chemicals and less than 0.1% carcinogenic chemicals. All potential hazards are listed below: α-target antibody, α-phospho-target antibody, α-GAPDH antibody: < 0.1% Sodium azide 10X TBS: 1% Kathon CG/ICP 15X Wash buffer: 0.3% Kathon CG/ICP, 1.5% Tween-20 HRP-conjugated antibodies: 0.2% Kathon CG/ICP, 0.1% Tween-20 SDS solution: 1% Sodium Dodecyl Sulfate (SDS) Blocking buffer: 0.5% Triton X-100, 0.1% Tween-20 Primary antibody diluent: 1% Kathon CG/ICP, 0.5% Triton X-100, 0.1% Tween-20 Quenching buffer: 1% Hydrogen peroxide (H2O2) Crystal violet solution: 0.05% crystal violet Ready-to-use substrate: < 0.02% hydrogen peroxide and < 0.1% 3,3’,5,5’- Tetramethylbenzidine (TMB) Stop solution: 2N Sulfuric acid (H2SO4)

2.1 Classification of the substance or mixture

Crystal violet: Carcinogen (2); Eye and skin irritant; Flammable liquid; Toxic by inhalation, ingestion, and skin absorption); Organ Damage (Eyes, Heart, Kidney, Liver, Nerves). Hydrogen peroxide: Carcinogen; Corrosive; Flammable Oxidizer; Organ Damage (Eyes, Respiratory system, Skin); Toxic by ingestion. Kathon CG: Carcinogen; Corrosive; Flammable Oxidizer; Harmful by ingestion; Harmful if inhaled and extremely destructive of tissues of mucous membranes and upper respiratory tract; Organ Damage (Blood, Central Nervous System, Eye, Gastrointestinal tract, Kidney); Skin and respiratory sensitizer (causes allergies). SDS: Flammable Solid; Organ Damage (Eyes and Lungs); Skin Irritant; Toxic by ingestion and skin absorption. Sodium azide: May react with lead and copper plumbing to form highly explosive metal azides; Highly toxic by ingestion and skin absorption; Organ Damage (Brain, Central nervous system, Heart). Sulfuric acid: Corrosive; Organ Damage (Eyes, Lungs, and Teeth). Triton X-100: Toxic by ingestion; Organ Damage (Eyes); Skin Irritant. Tween-20: Toxic by inhalation, ingestion, and skin absorption. May cause eye, skin, or respiratory system irritation.

Signal word

Warning Danger

Hazard statements

Keep away from clothing and combustible materials. Avoid breathing the fumes and vapors from kit components. Wear protective gloves, clothing, eyewear, and face protection.

Principle Routes of Exposure

Avoid ingestion and inhalation; avoid contact with skin and eyes Threshold limit value and source: The chemical, physical and toxicological properties of these components have not been thoroughly investigated. Allergy-prone individuals should be particularly cautious with proteins or other materials of biological origin. Carcinogenic effects data: Not available Chronic effects of overexposure: Not available Solubility in water: Liquids except detergents are readily miscible in water.

2.3 Other hazards

none