QuikChange XL Site-Directed Mutagenesis Kit, Part Number 200517 : 200517

Hazard statements (CLP)

H227, H317, H318, H319, H320, H361, H373, H412

Code

Statements

H227

Combustible liquid

SECTION 2: Hazards identification

OSHA Hazards Classification of the substance or mixture

Hazard pictograms

10X Reaction Buffer Dpn I XL10-Gold 2-Mercaptoethanol

Signal word

QuikSolution Warning XL10-Gold Ultracompetent cells Warning pUC 18 DNA Control Plasmid No signal word. PfuTurbo DNA Polymerase Warning 10X Reaction Buffer Warning Dpn I Warning Control Primer 1 (34-mer) No signal word. Control Primer 2 (34-mer) No signal word. pWS4.5 Control Template No signal word. QuikChange XL dNTP Mix No signal word. XL10-Gold 2-Mercaptoethanol Danger

Hazard statements

QuikSolution H227 - Combustible liquid. H320 - Causes eye irritation. XL10-Gold Ultracompetent cells H320 - Causes eye irritation. pUC 18 DNA Control Plasmid No known significant effects or critical hazards. PfuTurbo DNA Polymerase H320 - Causes eye irritation. 10X Reaction Buffer H319 - Causes serious eye irritation. Dpn I H319 - Causes serious eye irritation. Control Primer 1 (34-mer) No known significant effects or critical hazards. Control Primer 2 (34-mer) No known significant effects or critical hazards. pWS4.5 Control Template No known significant effects or critical hazards. QuikChange XL dNTP Mix No known significant effects or critical hazards. XL10-Gold 2-Mercaptoethanol H317 - May cause an allergic skin reaction. H318 - Causes serious eye damage. H361 - Suspected of damaging fertility or the unborn child. H373 - May cause damage to organs through prolonged or repeated exposure. (heart, liver) H412 - Harmful to aquatic life with long lasting effects.

Prevention

QuikSolution P210 - Keep away from flames and hot surfaces. No smoking. XL10-Gold Ultracompetent cells Not applicable. pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer P280 - Wear eye or face protection. Dpn I P280 - Wear eye or face protection. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold 2-Mercaptoethanol P280 - Wear protective gloves, protective clothing and eye or face protection. P260 - Do not breathe vapor.

Response

QuikSolution P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. XL10-Gold Ultracompetent cells P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. 10X Reaction Buffer P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. Dpn I P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold 2-Mercaptoethanol P363 - Wash contaminated clothing before reuse. P305 + P351 + P338, P310 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor.

Storage

QuikSolution P403 + P235 - Store in a well-ventilated place. Keep cool. XL10-Gold Ultracompetent cells Not applicable. pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer Not applicable. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold 2-Mercaptoethanol Not applicable.

Disposal

QuikSolution P501 - Dispose of contents and container in accordance with all local, regional, national and international regulations. XL10-Gold Ultracompetent cells Not applicable. pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer Not applicable. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold 2-Mercaptoethanol P501 - Dispose of contents and container in accordance with all local, regional, national and international regulations.

Supplemental label elements

QuikSolution None known. elements XL10-Gold Ultracompetent cells None known. pUC 18 DNA Control Plasmid None known. PfuTurbo DNA Polymerase None known. 10X Reaction Buffer None known. Dpn I None known. Control Primer 1 (34-mer) None known. Control Primer 2 (34-mer) None known. pWS4.5 Control Template None known. QuikChange XL dNTP Mix None known. XL10-Gold 2-Mercaptoethanol None known.

2.3 Other hazards

Hazards not otherwise QuikSolution None known. classified XL10-Gold Ultracompetent cells None known. pUC 18 DNA Control Plasmid None known. PfuTurbo DNA Polymerase None known. 10X Reaction Buffer None known. Dpn I None known. Control Primer 1 (34-mer) None known. Control Primer 2 (34-mer) None known. pWS4.5 Control Template None known. QuikChange XL dNTP Mix None known. XL10-Gold 2-Mercaptoethanol None known.

2.1 Classification of the substance or mixture

OSHA/HCS status : QuikSolution This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). XL10-Gold Ultracompetent This material is considered hazardous by the OSHA cells Hazard Communication Standard (29 CFR 1910.1200). pUC 18 DNA Control While this material is not considered hazardous by the Plasmid OSHA Hazard Communication Standard (29 CFR 1910.1200), this SDS contains valuable information critical to the safe handling and proper use of the product. This SDS should be retained and available for employees Section 2. Hazards identification and other users of this product. PfuTurbo DNA Polymerase This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 10X Reaction Buffer This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Dpn I This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Control Primer 1 (34-mer) While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this SDS contains valuable information critical to the safe handling and proper use of the product. This SDS should be retained and available for employees and other users of this product. Control Primer 2 (34-mer) While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this SDS contains valuable information critical to the safe handling and proper use of the product. This SDS should be retained and available for employees and other users of this product. pWS4.5 Control Template While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this SDS contains valuable information critical to the safe handling and proper use of the product. This SDS should be retained and available for employees and other users of this product. QuikChange XL dNTP Mix While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this SDS contains valuable information critical to the safe handling and proper use of the product. This SDS should be retained and available for employees and other users of this product. XL10-Gold This material is considered hazardous by the OSHA 2-Mercaptoethanol Hazard Communication Standard (29 CFR 1910.1200). Classification of the substance or mixture QuikSolution H227 FLAMMABLE LIQUIDS - Category 4 H320 EYE IRRITATION - Category 2B XL10-Gold Ultracompetent cells H320 EYE IRRITATION - Category 2B PfuTurbo DNA Polymerase H320 EYE IRRITATION - Category 2B 10X Reaction Buffer H319 EYE IRRITATION - Category 2A Dpn I H319 EYE IRRITATION - Category 2A XL10-Gold 2-Mercaptoethanol H318 SERIOUS EYE DAMAGE - Category 1 H317 SKIN SENSITIZATION - Category 1 H361 TOXIC TO REPRODUCTION - Category 2 H373 SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) - Category 2 H412 AQUATIC HAZARD (LONG-TERM) - Category 3 Section 2. Hazards identification QuikChange XL dNTP Mix Percentage of the mixture consisting of ingredient (s) of unknown hazards to the aquatic environment: 5.7%