QuikChange XL Site-Directed Mutagenesis Kit, Part Number 200516

Hazard statements (CLP)

H227, H317, H318, H319, H361, H412

Code

Statements

H227

Combustible liquid

H317

May cause an allergic skin reaction

H318

Causes serious eye damage.

H319

Causes serious eye irritation

H361

Suspected of damaging fertility or the unborn child

H412

Harmful to aquatic life with long lasting effects

2.1 Classification of the substance or mixture

QuikSolution H227 FLAMMABLE LIQUIDS - Category 4 10X Reaction Buffer H319 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2A XL10-Gold 2-Mercaptoethanol H318 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 1 H317 SKIN SENSITISATION - Category 1 H361 REPRODUCTIVE TOXICITY - Category 2 H412 LONG-TERM (CHRONIC) AQUATIC HAZARD - Category 3 QuikChange XL dNTP Mix Percentage of the mixture consisting of ingredient(s) of unknown hazards to the aquatic environment: 5.7%

Hazard pictograms

10X Reaction Buffer XL10-Gold 2-Mercaptoethanol

Signal word

QuikSolution WARNING XL10-Gold Ultracompetent No signal word. cells pUC 18 DNA Control Plasmid No signal word. PfuTurbo DNA Polymerase No signal word. 10X Reaction Buffer WARNING Dpn I No signal word. Control Primer 1 (34-mer) No signal word. Control Primer 2 (34-mer) No signal word. pWS4.5 Control Template No signal word. QuikChange XL dNTP Mix No signal word. XL10-Gold DANGER 2-Mercaptoethanol

Hazard statements

QuikSolution H227 - Combustible liquid. XL10-Gold Ultracompetent No known significant effects or critical hazards. cells pUC 18 DNA Control Plasmid No known significant effects or critical hazards. PfuTurbo DNA Polymerase No known significant effects or critical hazards. 10X Reaction Buffer H319 - Causes serious eye irritation. Dpn I No known significant effects or critical hazards. Control Primer 1 (34-mer) No known significant effects or critical hazards. Control Primer 2 (34-mer) No known significant effects or critical hazards. pWS4.5 Control Template No known significant effects or critical hazards. QuikChange XL dNTP Mix No known significant effects or critical hazards. XL10-Gold H317 - May cause an allergic skin reaction. 2-Mercaptoethanol H318 - Causes serious eye damage. H361 - Suspected of damaging fertility or the unborn child. H412 - Harmful to aquatic life with long lasting effects.

Prevention

QuikSolution P210 - Keep away from flames and hot surfaces. No smoking. XL10-Gold Ultracompetent Not applicable. cells pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer P280 - Wear eye or face protection. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold P201 - Obtain special instructions before use. 2-Mercaptoethanol P281 - Use personal protective equipment as required. P280 - Wear protective gloves. Wear eye or face protection.

Response

QuikSolution Not applicable. XL10-Gold Ultracompetent Not applicable. cells pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold P305 + P351 + P338, P310 - IF IN EYES: Rinse 2-Mercaptoethanol cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor.

Storage

QuikSolution P403 + P235 - Store in a well-ventilated place. Keep cool. XL10-Gold Ultracompetent Not applicable. cells pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer Not applicable. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold Not applicable. 2-Mercaptoethanol

Disposal

QuikSolution P501 - Dispose of contents and container in accordance with all local, regional, national and international regulations. XL10-Gold Ultracompetent Not applicable. cells pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer Not applicable. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold P501 - Dispose of contents and container in 2-Mercaptoethanol accordance with all local, regional, national and international regulations.

Supplemental label elements

elements Additional warning : QuikSolution Not applicable. phrases XL10-Gold Ultracompetent Not applicable. cells pUC 18 DNA Control Plasmid Not applicable. PfuTurbo DNA Polymerase Not applicable. 10X Reaction Buffer Not applicable. Dpn I Not applicable. Control Primer 1 (34-mer) Not applicable. Control Primer 2 (34-mer) Not applicable. pWS4.5 Control Template Not applicable. QuikChange XL dNTP Mix Not applicable. XL10-Gold Not applicable. 2-Mercaptoethanol Other hazards which do not : QuikSolution None known. result in classification XL10-Gold Ultracompetent None known. cells pUC 18 DNA Control Plasmid None known. PfuTurbo DNA Polymerase None known. 10X Reaction Buffer None known. Dpn I None known. Control Primer 1 (34-mer) None known. Control Primer 2 (34-mer) None known. pWS4.5 Control Template None known. QuikChange XL dNTP Mix None known. XL10-Gold None known. 2-Mercaptoethanol