GeneMorph II EZClone Domain Mutagenesis Kit, Part Number 200552

Hazard statements (CLP)

H227, H317, H318, H361, H412

Code

Statements

H227

Combustible liquid

2.1 Classification of the substance or mixture

EZClone Solution H227 FLAMMABLE LIQUIDS - Category 4 XL10-Gold 2-Mercaptoethanol H318 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 1 H317 SKIN SENSITISATION - Category 1 H361 REPRODUCTIVE TOXICITY - Category 2 H412 LONG-TERM (CHRONIC) AQUATIC HAZARD - Category 3 10X Mutazyme II Reaction Percentage of the mixture consisting of ingredient(s) Buffer of unknown hazards to the aquatic environment: 9% 40 mM dNTP Mix (10 mM Percentage of the mixture consisting of ingredient(s) dNTP) of unknown hazards to the aquatic environment: 4.2%

Hazard pictograms

XL10-Gold 2-Mercaptoethanol

Signal word

EZClone Solution WARNING Mutazyme II No signal word. 10X Mutazyme II Reaction No signal word. Buffer 40 mM dNTP Mix (10 mM No signal word. dNTP) 2X EZClone Enzyme Mix No signal word. Dpn I No signal word. Positive Control Plasmid No signal word. Positive Control Primer Mix No signal word. 1.1 kb Gel Standard No signal word. XL10-Gold Ultracompetent No signal word. cells XL10-Gold DANGER 2-Mercaptoethanol pUC 18 DNA Control Plasmid No signal word.

Hazard statements

EZClone Solution H227 - Combustible liquid. Mutazyme II No known significant effects or critical hazards. 10X Mutazyme II Reaction No known significant effects or critical hazards. Buffer 40 mM dNTP Mix (10 mM No known significant effects or critical hazards. dNTP) 2X EZClone Enzyme Mix No known significant effects or critical hazards. Dpn I No known significant effects or critical hazards. Positive Control Plasmid No known significant effects or critical hazards. Positive Control Primer Mix No known significant effects or critical hazards. 1.1 kb Gel Standard No known significant effects or critical hazards. XL10-Gold Ultracompetent No known significant effects or critical hazards. cells XL10-Gold H317 - May cause an allergic skin reaction. 2-Mercaptoethanol H318 - Causes serious eye damage. H361 - Suspected of damaging fertility or the unborn child. H412 - Harmful to aquatic life with long lasting effects. pUC 18 DNA Control Plasmid No known significant effects or critical hazards.

Prevention

EZClone Solution P210 - Keep away from flames and hot surfaces. No smoking. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold P201 - Obtain special instructions before use. 2-Mercaptoethanol P281 - Use personal protective equipment as required. P280 - Wear protective gloves. Wear eye or face protection. pUC 18 DNA Control Plasmid Not applicable.

Response

EZClone Solution Not applicable. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold P305 + P351 + P338, P310 - IF IN EYES: Rinse 2-Mercaptoethanol cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor. pUC 18 DNA Control Plasmid Not applicable.

Storage

EZClone Solution P403 + P235 - Store in a well-ventilated place. Keep cool. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold Not applicable. 2-Mercaptoethanol pUC 18 DNA Control Plasmid Not applicable.

Disposal

EZClone Solution P501 - Dispose of contents and container in accordance with all local, regional, national and international regulations. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold P501 - Dispose of contents and container in 2-Mercaptoethanol accordance with all local, regional, national and international regulations. pUC 18 DNA Control Plasmid Not applicable.

Supplemental label elements

elements Additional warning : EZClone Solution Not applicable. phrases Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold Not applicable. 2-Mercaptoethanol pUC 18 DNA Control Plasmid Not applicable. Other hazards which do not : EZClone Solution None known. result in classification Mutazyme II None known. 10X Mutazyme II Reaction None known. Buffer 40 mM dNTP Mix (10 mM None known. dNTP) 2X EZClone Enzyme Mix None known. Dpn I None known. Positive Control Plasmid None known. Positive Control Primer Mix None known. 1.1 kb Gel Standard None known. XL10-Gold Ultracompetent None known. cells XL10-Gold None known. 2-Mercaptoethanol pUC 18 DNA Control Plasmid None known.