GeneMorph II EZClone Domain Mutagenesis Kit, Part Number 200552

Hazard statements (CLP)

H227, H317, H318, H320, H361, H412

Code

Statements

H227

Combustible liquid

SECTION 2: Hazards identification

Classification of the substance or mixture EZClone Solution H227 FLAMMABLE LIQUIDS - Category 4 H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B Mutazyme II H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B Dpn I H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B XL10-Gold Ultracompetent cells H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B XL10-Gold 2-Mercaptoethanol H318 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 1 H317 SKIN SENSITISATION - Category 1 H361 REPRODUCTIVE TOXICITY - Category 2 H412 LONG-TERM (CHRONIC) AQUATIC HAZARD - Category 3 10X Mutazyme II Reaction Percentage of the mixture consisting of ingredient(s) Buffer of unknown hazards to the aquatic environment: 9% 40 mM dNTP Mix (10 mM Percentage of the mixture consisting of ingredient(s) dNTP) of unknown hazards to the aquatic environment: 4.2% XL10-Gold Ultracompetent Percentage of the mixture consisting of ingredient(s) cells of unknown hazards to the aquatic environment: 5%

Hazard pictograms

XL10-Gold 2-Mercaptoethanol

Signal word

EZClone Solution WARNING Mutazyme II WARNING 10X Mutazyme II Reaction No signal word. Buffer 40 mM dNTP Mix (10 mM No signal word. dNTP) 2X EZClone Enzyme Mix No signal word. Dpn I WARNING Positive Control Plasmid No signal word. Positive Control Primer Mix No signal word. 1.1 kb Gel Standard No signal word. XL10-Gold Ultracompetent WARNING cells XL10-Gold DANGER 2-Mercaptoethanol pUC 18 DNA Control Plasmid No signal word.

Hazard statements

EZClone Solution H227 - Combustible liquid. H320 - Causes eye irritation. Mutazyme II H320 - Causes eye irritation. 10X Mutazyme II Reaction No known significant effects or critical hazards. Buffer 40 mM dNTP Mix (10 mM No known significant effects or critical hazards. dNTP) 2X EZClone Enzyme Mix No known significant effects or critical hazards. Dpn I H320 - Causes eye irritation. Positive Control Plasmid No known significant effects or critical hazards. Positive Control Primer Mix No known significant effects or critical hazards. 1.1 kb Gel Standard No known significant effects or critical hazards. XL10-Gold Ultracompetent H320 - Causes eye irritation. cells XL10-Gold H317 - May cause an allergic skin reaction. 2-Mercaptoethanol H318 - Causes serious eye damage. H361 - Suspected of damaging fertility or the unborn child. H412 - Harmful to aquatic life with long lasting effects. pUC 18 DNA Control Plasmid No known significant effects or critical hazards.

Prevention

EZClone Solution P210 - Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold P201 - Obtain special instructions before use. 2-Mercaptoethanol P280 - Wear protective gloves, protective clothing and eye or face protection. P273 - Avoid release to the environment. pUC 18 DNA Control Plasmid Not applicable.

Response

EZClone Solution P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P337 + P313 - If eye irritation persists: Get medical advice or attention. Mutazyme II P305 + P351 + P338 - IF IN EYES: Rinse cautiously 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I P305 + P351 + P338 - IF IN EYES: Rinse cautiously Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent P305 + P351 + P338 - IF IN EYES: Rinse cautiously cells with water for several minutes. Remove contact XL10-Gold P305 + P351 + P338, P310 - IF IN EYES: Rinse 2-Mercaptoethanol cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor. pUC 18 DNA Control Plasmid Not applicable.

Storage

EZClone Solution Not applicable. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold Not applicable. 2-Mercaptoethanol pUC 18 DNA Control Plasmid Not applicable.

Disposal

EZClone Solution P501 - Dispose of contents and container in accordance with all local, regional, national and international regulations. Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold P501 - Dispose of contents and container in 2-Mercaptoethanol accordance with all local, regional, national and pUC 18 DNA Control Plasmid Not applicable.

Supplemental label elements

Additional warning : EZClone Solution Not applicable. phrases Mutazyme II Not applicable. 10X Mutazyme II Reaction Not applicable. Buffer 40 mM dNTP Mix (10 mM Not applicable. dNTP) 2X EZClone Enzyme Mix Not applicable. Dpn I Not applicable. Positive Control Plasmid Not applicable. Positive Control Primer Mix Not applicable. 1.1 kb Gel Standard Not applicable. XL10-Gold Ultracompetent Not applicable. cells XL10-Gold Not applicable. 2-Mercaptoethanol pUC 18 DNA Control Plasmid Not applicable.

Other hazards which do not result in classification

EZClone Solution None known. Mutazyme II None known. 10X Mutazyme II Reaction None known. Buffer 40 mM dNTP Mix (10 mM None known. dNTP) 2X EZClone Enzyme Mix None known. Dpn I None known. Positive Control Plasmid None known. Positive Control Primer Mix None known. 1.1 kb Gel Standard None known. XL10-Gold Ultracompetent None known. cells XL10-Gold None known. 2-Mercaptoethanol pUC 18 DNA Control Plasmid None known.

2.1 Classification of the substance or mixture

EZClone Solution H227 FLAMMABLE LIQUIDS - Category 4 H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B Mutazyme II H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B Dpn I H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B XL10-Gold Ultracompetent Section 2. Hazard(s) identification cells H320 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 2B XL10-Gold 2-Mercaptoethanol H318 SERIOUS EYE DAMAGE/EYE IRRITATION - Category 1 H317 SKIN SENSITISATION - Category 1 H361 REPRODUCTIVE TOXICITY - Category 2 H412 LONG-TERM (CHRONIC) AQUATIC HAZARD - Category 3 10X Mutazyme II Reaction Percentage of the mixture consisting of ingredient(s) Buffer of unknown hazards to the aquatic environment: 9% 40 mM dNTP Mix (10 mM Percentage of the mixture consisting of ingredient(s) dNTP) of unknown hazards to the aquatic environment: 4.2% XL10-Gold Ultracompetent Percentage of the mixture consisting of ingredient(s) cells of unknown hazards to the aquatic environment: 5%